Pliuta (Plyuta) Vladimir Alexandrovich | Institute of Molecular Genetics
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Academic degree:
Doctor of Philosophy (Biology)
Academic title:
without academic rank
Division of IMG:
Laboratory regulation of gene expression of microorganisms
Position:
Researcher
Telephone:
+7-499-196-02-15
Е-mail: |
Main research interests
1) Volatile organic compounds synthesized by bacteria: nature, molecular genetic and functional studies.
2013-2015. The work is devoted to research of volatile organic compounds (VOCs), synthesized by bacteria, studying of their nature and mechanisms of action.
Rhizosphere bacterial strains are used as the main objects-producers of VOCs. Priority data were obtained in studies of the effects of gas mixtures of volatile substances produced by bacterial strains belonging to the genera Pseudomonas and Serratia, and individual VOCs - dimethyl disulfide (DMDS) and ketones on formation and mature biofilm Agrobacterium, pathogens of tumor formation in plants. It was shown that the total pool of volatile substances formed by these bacteria, and individual VOCs inhibit the biofilms formation of A. tumefaciens and kill bacteria in mature biofilms. It is shown that the pool VOC formed by these bacteria and separately DMDS and ketones inhibit the functioning of Quorum Sensing regulation systems of different bacteria at concentrations that do not affect their growth. These results open up new aspects of the competitive relationship between microorganisms and the relationship of bacteria and plants. These data are significant for the development of methods of biological control of plant diseases with the use of bacteria-antagonists of plant pathogens.
2. Bacterial biofilms: laws of biofilm formation and the impact of different compounds on biofilms and their formation, interconnection functioning of Quorum Sensing system of regulation of gene expression and the formation of bacterial biofilms.
2009—2013. The work deals with researching the laws of the action on bacterial biofilm formation substances of plant origin (natural phenolic compounds with antimicrobial activity and plant hormones), oxidants, as well as the study of interconnection functioning Quorum Sensing (QS) systems and formation of bacterial biofilms under the influence of these substances. It is shown that the phenolic compounds of plant origin and phytohormones - salicylic, indoleacetic, abscisic and gibberellic acids at subinhibitory or slightly inhibitory the growth of bacteria concentrations stimulate the formation of biofilms by these bacteria (Plyuta V. et al. APMIS. 2013. V. 121. P.1073-1081; Plyuta V. et al. Applied Biochemistry and Microbiology. 2013. V. 49. P. 706-710). Under the action of low concentrations of phenolic compounds also occurs increase synthesis of N-3-oxo-dodecanoyl-homoserinelactone - signaling molecule of major Las QS system of Pseudomonas aeruginosa. These data suggest that stimulation of biofilm formation and functioning of QS system P. aeruginosa are interconnected. Under the action of subinhibitory concentrations of hydrogen peroxide on the cells of P. aeruginosa also was occurred the stimulation of biofilm formation; the introduction of the heterologous gene aiiA encoding the N-acyl-homoserine lactonase AiiA (enzyme that degrading AHL) into the cells removes this effect. These data indicates that the stimulation of the biofilm formation P. aeruginosa PAO1 in the presence of H2O2 depends on the functioning of the QS systems of the gene expression regulation of this bacteria (Плюта В.А. и др. Молекулярная генетика, микробиология и вирусология. 2013. № 4: С. 10-14.).
2015. The defense of Ph.D. dissertation work on the topic of "Peculiarities of biofilm formation and Quorum Sensing regulation under the action of antibacterial agents" in the specialties: 03.01.06 - Biotechnology (including bionanotechnology) and 03.02.03 - microbiology.
2013-2015. The work is devoted to research of volatile organic compounds (VOCs), synthesized by bacteria, studying of their nature and mechanisms of action.
Rhizosphere bacterial strains are used as the main objects-producers of VOCs. Priority data were obtained in studies of the effects of gas mixtures of volatile substances produced by bacterial strains belonging to the genera Pseudomonas and Serratia, and individual VOCs - dimethyl disulfide (DMDS) and ketones on formation and mature biofilm Agrobacterium, pathogens of tumor formation in plants. It was shown that the total pool of volatile substances formed by these bacteria, and individual VOCs inhibit the biofilms formation of A. tumefaciens and kill bacteria in mature biofilms. It is shown that the pool VOC formed by these bacteria and separately DMDS and ketones inhibit the functioning of Quorum Sensing regulation systems of different bacteria at concentrations that do not affect their growth. These results open up new aspects of the competitive relationship between microorganisms and the relationship of bacteria and plants. These data are significant for the development of methods of biological control of plant diseases with the use of bacteria-antagonists of plant pathogens.
2. Bacterial biofilms: laws of biofilm formation and the impact of different compounds on biofilms and their formation, interconnection functioning of Quorum Sensing system of regulation of gene expression and the formation of bacterial biofilms.
2009—2013. The work deals with researching the laws of the action on bacterial biofilm formation substances of plant origin (natural phenolic compounds with antimicrobial activity and plant hormones), oxidants, as well as the study of interconnection functioning Quorum Sensing (QS) systems and formation of bacterial biofilms under the influence of these substances. It is shown that the phenolic compounds of plant origin and phytohormones - salicylic, indoleacetic, abscisic and gibberellic acids at subinhibitory or slightly inhibitory the growth of bacteria concentrations stimulate the formation of biofilms by these bacteria (Plyuta V. et al. APMIS. 2013. V. 121. P.1073-1081; Plyuta V. et al. Applied Biochemistry and Microbiology. 2013. V. 49. P. 706-710). Under the action of low concentrations of phenolic compounds also occurs increase synthesis of N-3-oxo-dodecanoyl-homoserinelactone - signaling molecule of major Las QS system of Pseudomonas aeruginosa. These data suggest that stimulation of biofilm formation and functioning of QS system P. aeruginosa are interconnected. Under the action of subinhibitory concentrations of hydrogen peroxide on the cells of P. aeruginosa also was occurred the stimulation of biofilm formation; the introduction of the heterologous gene aiiA encoding the N-acyl-homoserine lactonase AiiA (enzyme that degrading AHL) into the cells removes this effect. These data indicates that the stimulation of the biofilm formation P. aeruginosa PAO1 in the presence of H2O2 depends on the functioning of the QS systems of the gene expression regulation of this bacteria (Плюта В.А. и др. Молекулярная генетика, микробиология и вирусология. 2013. № 4: С. 10-14.).
2015. The defense of Ph.D. dissertation work on the topic of "Peculiarities of biofilm formation and Quorum Sensing regulation under the action of antibacterial agents" in the specialties: 03.01.06 - Biotechnology (including bionanotechnology) and 03.02.03 - microbiology.
Publications
- Плюта В.А., Попова А.А., Кокшарова О.А., Кузнецов А.Е., Хмель И.А. Способность природных кетонов взаимодействовать с бактериальными Quorum Sensing системами // Молекулярная генетика, микробиология и вирусология. 2014. № 4: С. 10-13.
- Plyuta V., Zaitseva J., Lobakova E., Zagoskina N., Kuznetsov A., Khmel I. Effect of plant phenolic compounds on biofilm formation by Pseudomonas aeruginosa // APMIS. 2013. V. 121. P.1073-1081.
- Plyuta V. A., Lipasova V. A., Kuznetsov, A. E., Khmel I. A. Effect of salicylic, indole-3-acetic, gibberellic, and abscisic acids on biofilm formation by Agrobacterium tumefaciens C58 and Pseudomonas aeruginosa PAO1 // Applied Biochemistry and Microbiology. 2013. V. 49. P. 706-710.
- Плюта В.А., Андреенко Ю.В., Кузнецов А.Е., Хмель И.А. Образование биопленок Pseudomonas aeruginosa РАО1 в присутствии перекиси водорода; влияние гена aiiA // Молекулярная генетика, микробиология и вирусология. 2013. № 4: С. 10-14.
- Плюта В.А, Липасова В.А., Кузнецов А.Е., Хмель И.А. Действие салициловой, индолил-3-уксусной, гиббереллиновой и абсцизовой кислот на образование биопленок бактериями Agrobacterium tumefaciens C58 и Pseudomonas aeruginosa PAO1 // Биотехнология. 2012. № 3: C. 53-58.