Makarova Alena Vladimirovna | Institute of Molecular Genetics
Academic degree:
Doctor of Philosophy (Biology)
Academic title:
without academic rank
Division of IMG:
Laboratory “Mechanisms of Replication of damaged DNA”
Position:
Head of Laboratory
Telephone:
+7-499-196-00-15
Е-mail: |
Main research interests
Fidelity of replication and mutagenesis by human specialized DNA polymerases (Pol iota, Pol eta, Pol zeta, PrimPol), translesion DNA synthesis.
Grants (Principal Investigator)
Studies are supported by:
- RFBR 17-00-00264-комфи. 2018-2020 (together with D. Zharkov and A. Rizvanov)
- RSF 18-14-00354, 2018-2020
- RFBR 19-34-90147, 2019-2021 (grant for PhD students)
- RFBR 18-04-00777-а, 2018-2020
- RFBR 18-54-00024-Бел-а, 2018-2019
Completed Grant Projects:
- RFBR 12-04-01697-а. 2012-2014.
- RFBR 15-04-08398-а. 2015-2017.
- RFBR and The Government of Moscow 15-34-70002-мол_а_мос, 2016-2017.
- Program of The Presidium of RAS «Molecular and Cellular Biology. New groups», 2015-2018.
Teaching activities
Boldinova Elizaveta, PhD student.
Shilkin Evgeniy, PhD student.
Gagarinskaya Diana, PhD student.
Awards, achievements, memberships
1. Postdoctoral fellowship of D. Zimin foundation “Dynasty”, 2014-2016.
2. Fellowship of the President of Russian Federation for Young Scientists, 2016-2017.
3. The L'Oréal-UNESCO For Women in Science (national fellowship, Moscow. Russia), 2017
Publications
- Yudkina A.V., Shilkin E.S., Endutkin A.V., Makarova A.V.*, Zharkov D.O.* Reading and misreading 8-oxoguanine, a paradigmatic ambiguous nucleobase // Crystals, 2019, 9(5): 269, *corresponding authors
- Kim D.V., Makarova A.V., Miftakhova R.R. Zharkov D.O. Base excision DNA repair deficient cells: from disease models to genotoxicity sensors // Curr Pharm Design, 2019, 25(3): 298 — 312
- Boldinova E.O., Khairullin R.K., Makarova A.V.*, Zharkov D.O.* Isoforms of base excision repair enzymes produced by alternative splicing // Int J Mol Sc., 2019, 20(13). pii: E3279. *corresponding authors
- Gagarinskaya D.I., Makarova A.V. A multifunctional protein PolDIP2 in DNA translesion synthesis // Adv Exp Med Biol, 2019, in press
- Boldinova E.O., Ignatov A.V., Kulbachinskiy A.V., Makarova A.V. The active site residues Gln55 and Arg73 play a key role in DNA damage bypass by S. cerevisiae Pol η // Scientific Reports, 2018, 8 (1), 1031
- Makarova A.V.*, Boldinova E.O., Belousova E.A., Lavrik O.I.* In vitro lesion bypass by human PrimPol // DNA Repair, 2018, 70:18-24
- Miropolskaya N., Petushkov I., Kulbachinskiy A., Makarova A.V. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι // Scientific Reports 2017, 7, 10194
- Kazachenko K.Y., Miropolskaya N.A., Gening L.G., Tarantul V.Z., Makarova A.V. Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro // DNA Repair, 2017, 50:77-82.
- Boldinova E.O., Wanrooij P.H., Shilkin E.S., Wanrooij S.*, Makarova A.V.* DNA damage tolerance by eukaryotic DNA polymerase and primase PrimPol // Int. J. Mol. Sci. 2017, 18(7), pii: E1584. *corresponding authors
- Boldinova E.O., Stojkovič G., Khairullin R., Wanrooij S.*, Makarova A.V.* Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol // PLoS One, 2017, PLoS ONE 12(9): e0184489. *corresponding authors
- Ignatov A.V., Bondarenko K.A., Makarova A.V. Non-bulky Lesions in Human DNA: the Ways of Formation, Repair, and Replication // Acta Naturae 2017, 9(3):12-26.
- Kochenova O.V., Bezalel-Buch R., Tran P., Makarova A.V., Chabes A., Burgers P.M., Shcherbakova P.V. Yeast DNA polymerase ζ maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations // Nucleic Acids Research, 2017, 45(3):1200-1218
- Sawicka M., Wanrooij P.H., Darbari V.C., Tannous E., Hailemariam S., Bose D., Makarova A.V., Burgers P.M., Zhang X. The dimeric architecture of checkpoint kinases Mec1/ATR and Tel1/ATM reveal a common structural organisation // J Biol Chem, 2016: 291,13436-13447.
- Stojkovič G.*, Makarova A.V*., Wanrooij P.H., Forslund J., Burgers P.M., Wanrooij S. Oxidative DNA damage stalls the human mitochondrial replisome // Scientific Reports, 2016: 6, 28942. * equal contribution
- Makarova A.V. and Burgers P.M. Eukaryotic DNA polymerase zeta // DNA Repair, 2015: 29, 47-55. (review)
- Makarova A.V.*, Ignatov A., Miropolskaya N, Kulbachinskiy A.* Roles of the active site residues and metal cofactors in noncanonical base-pairing during catalysis by human DNA polymerase iota // DNA Repair, 2014, 22: 67-76. *corresponding authors
- Makarova A.V. and Burgers P. “DNA polymerase ζ and Mutagenesis in Eukaryotic Cells,.”, in “Translesion DNA polymerases: from DNA repair and beyond”, Maiorano D. and Hoffmann J-S., Eds.: Research Signpost, 2015, 153-177. (chapter in a book).
- Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Error-prone Replication Bypass of the Primary Aflatoxin B1 DNA Adduct, AFB1-N7-Gua // J Biol Chem, 2014: 289(26), 18497-18506.
- Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Molecular basis of aflatoxin-induced mutagenesis – role of the aflatoxin B1-formamidopyrimidine adduct // Carcinogenesis, 2014: 35(7), 1461-1468.
- Makarova A.V., Nick McElhinny S.A., Watts B.E., Kunkel T.A., Burgers P.M. Ribonucleotide incorporation by yeast DNA polymerase ζ // DNA Repair, 2014: 18, 63-67.
- Makarova A.V., Stodola J.L., Burgers P.M. A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis // Nucleic Acids Research, 2012: 40(22), 11618 – 11626.
- Smith L.A.*, Makarova A.V.*, Samson L., Thiesen K.E., Dhar A., Bessho T. Bypass of a psoraen DNA interstrand cross-link by DNA polymerases beta, iota, and kappa in vitro // Biochemistry, 2012:51, 8931 – 8938. * equal contribution
- Makarova A.V., Kulbachinskiy A.V. Structure of human DNA polymerase iota and the mechanism of DNA synthesis // Biochemistry (Mosc). 2012, 77(6):547-61. *corresponding authors
- Lachin A.V., Kazakov A.A., Makarova A.V., Pavlov Y.I., Efremova A.S., Shram S.I., Тarantul V.Z., Gening L.V. Isolation and characterization of high affinity aptamers selected against DNA polymerase iota // Nucleic Acid Ther., 2012: 22 (1), 49 – 57.
- Makarova I.V., Kazakov A.A., Makarova A.V., Khaidarova N.V., Kozikova L.V., Nenasheva V.N., Gening L.V., Tarantul V.Z., Andreeva L.E. Transient expression and activity of human DNA polymerase iota in loach embryos // Biotechnology Letters, 2012: 34 (2), 205 – 212.
- Makarova A.V., Grabow C., Gening L.V., Tarantul V.Z., Tahirov T.T., Bessho T. Pavlov Y.I. Inaccurate DNA synthesis due to the presence of DNA polymerase iota activity in whole cell extracts // PLOS One, 2011: 6 (1), 1 – 10.
- Kazakov A.A., Makarova A.V., Grishina E.E., Tarantul V.Z., Gening L.V. Activity of DNA polymerase iota in human basal cell carcinoma // Curr. Topics Biochem. Res, 2010: 12 (2), 39 – 49.
- Макарова А.В., Генинг Л.В., Макарова И.В., Тарантул В.З. Активность склонной к ошибкам ДНК-полимеразы йота в разные периоды отногенеза домовой мыши Mus musculus // Онтогенез, 2008: 39(5), 367 – 373.
- Макарова А.В., Генинг Л.В., Тарантул В.З. Эволюция структуры и функции ДНК-полимеразы йота у эукариот // Биохимия, 2008: 73(3), 426 – 433.
- Генинг Л.В., Макарова А.В., Малашенко А.М., Тарантул В.З. Фальшивая нота ДНК-полимеразы йота в ансамбле защитников генома млекопитающих // Биохимия, 2006: 71(2), 155 – 159