Makarova Alena Vladimirovna | Institute of Molecular Genetics

Makarova Alena Vladimirovna

Academic degree:
Doctor of Philosophy (Biology)

Academic title:
without academic rank

Division of IMG:
Group "Specialized DNA polymerases"

Head of Group



Main research interests

Fidelity of replication and mutagenesis by human specialized DNA polymerases (Pol iota, Pol eta, Pol zeta, PrimPol), translesion DNA synthesis.

Teaching activities

Boldinova Elizaveta O., PhD student
Shilkin Evgeniy S. PhD student

Awards, achievements, memberships

1. Postdoctoral fellowship of D. Zimin foundation “Dynasty”, 2014-2016.
2. Fellowship of the President of Russian Federation for Young Scientists, 2016-2017.
3.  The L'Oréal-UNESCO For Women in Science (national fellowship, Moscow. Russia), 2017


  1. Makarova A.V.*, Boldinova E.O., Belousova E.A., Olga I. Lavrik O.I.* In vitro lesion bypass by human PrimPol // DNA Repair, on revision. *corresponding authors
  2. Boldinova E.O., Ignatov A.V., Kulbachinskiy A.V., Makarova A.V. The active site residues Gln55 and Arg73 play a key role in DNA damage bypass by S. cerevisiae Pol η // Scientific Reports, on revision.
  3. Miropolskaya N., Petushkov I., Kulbachinskiy A., Makarova A.V. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι // Scientific Reports 2017, 7, 10194
  4. Kazachenko K.Y., Miropolskaya N.A., Gening L.G., Tarantul V.Z., Makarova A.V. Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro // DNA Repair, 2017, 50:77-82.
  5. Boldinova E.O., Wanrooij P.H., Shilkin E.S., Wanrooij S.*, Makarova A.V.* DNA damage tolerance by eukaryotic DNA polymerase and primase PrimPol // Int. J. Mol. Sci. 2017, 18(7), pii: E1584. *corresponding authors
  6. Boldinova E.O., Stojkovič G., Khairullin R., Wanrooij S.*, Makarova A.V.* Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol // PLoS One, 2017, PLoS ONE 12(9): e0184489. *corresponding authors
  7. Ignatov A.V., Bondarenko K.A., Makarova A.V.  Non-bulky Lesions in Human DNA: the Ways of Formation, Repair, and Replication // Acta Naturae 2017, 9(3):12-26.
  8. Kochenova O.V., Bezalel-Buch R., Tran P., Makarova A.V., Chabes A., Burgers P.M., Shcherbakova P.V. Yeast DNA polymerase ζ maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations // Nucleic Acids Research, 2017, 45(3):1200-1218
  9. Sawicka M., Wanrooij P.H., Darbari V.C., Tannous E., Hailemariam S., Bose D., Makarova A.V., Burgers P.M., Zhang X. The dimeric architecture of checkpoint kinases Mec1/ATR and Tel1/ATM reveal a common structural organisation // J Biol Chem, 2016: 291,13436-13447.
  10. Stojkovič G.*, Makarova A.V*., Wanrooij P.H., Forslund J., Burgers P.M., Wanrooij S. Oxidative DNA damage stalls the human mitochondrial replisome // Scientific Reports, 2016: 6, 28942. * equal contribution
  11. Makarova A.V. and Burgers P.M. Eukaryotic DNA polymerase zeta // DNA Repair, 2015: 29, 47-55. (review)
  12. Makarova A.V.*, Ignatov A., Miropolskaya N, Kulbachinskiy A.* Roles of the active site residues and metal cofactors in noncanonical base-pairing during catalysis by human DNA polymerase iota // DNA Repair, 2014, 22: 67-76. *corresponding authors
  13. Makarova A.V. and Burgers P. “DNA polymerase ζ and Mutagenesis in Eukaryotic Cells,.”, in “Translesion DNA polymerases: from DNA repair and beyond”, Maiorano D. and Hoffmann J-S., Eds.: Research Signpost, 2015, 153-177. (chapter in a book).
  14. Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Error-prone Replication Bypass of the Primary Aflatoxin B1 DNA Adduct, AFB1-N7-Gua // J Biol Chem, 2014: 289(26), 18497-18506.
  15. Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Molecular basis of aflatoxin-induced mutagenesis – role of the aflatoxin B1-formamidopyrimidine adduct // Carcinogenesis, 2014: 35(7), 1461-1468.
  16. Makarova A.V., Nick McElhinny S.A., Watts B.E., Kunkel T.A., Burgers P.M. Ribonucleotide incorporation by yeast DNA polymerase ζ // DNA Repair, 2014: 18, 63-67.
  17. Makarova A.V., Stodola J.L., Burgers P.M. A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis // Nucleic Acids Research, 2012: 40(22), 11618 – 11626.
  18. Smith L.A.*, Makarova A.V.*, Samson L., Thiesen K.E., Dhar A., Bessho T. Bypass of a psoraen DNA interstrand cross-link by DNA polymerases beta, iota, and kappa in vitro // Biochemistry, 2012:51, 8931 – 8938. * equal contribution
  19. Makarova A.V., Kulbachinskiy A.V. Structure of human DNA polymerase iota and the mechanism of DNA synthesis // Biochemistry (Mosc). 2012, 77(6):547-61. *corresponding authors
  20. Lachin A.V., Kazakov A.A., Makarova A.V., Pavlov Y.I., Efremova A.S., Shram S.I., Тarantul V.Z., Gening L.V. Isolation and characterization of high affinity aptamers selected against DNA polymerase iota // Nucleic Acid Ther., 2012: 22 (1), 49 – 57.
  21. Makarova I.V., Kazakov A.A., Makarova A.V., Khaidarova N.V., Kozikova L.V., Nenasheva V.N., Gening L.V., Tarantul V.Z., Andreeva L.E. Transient expression and activity of human DNA polymerase iota in loach embryos // Biotechnology Letters, 2012: 34 (2), 205 – 212.
  22. Makarova A.V., Grabow C., Gening L.V., Tarantul V.Z., Tahirov T.T., Bessho T. Pavlov Y.I. Inaccurate DNA synthesis due to the presence of DNA polymerase iota activity in whole cell extracts // PLOS One, 2011: 6 (1), 1 – 10.
  23. Kazakov A.A., Makarova A.V., Grishina E.E., Tarantul V.Z., Gening L.V. Activity of DNA polymerase iota in human basal cell carcinoma // Curr. Topics Biochem. Res, 2010: 12 (2), 39 – 49.
  24. Макарова А.В., Генинг Л.В., Макарова И.В., Тарантул В.З. Активность склонной к ошибкам ДНК-полимеразы йота в разные периоды отногенеза домовой мыши Mus musculus // Онтогенез, 2008: 39(5), 367 – 373.
  25. Макарова А.В., Генинг Л.В., Тарантул В.З. Эволюция структуры и функции ДНК-полимеразы йота у эукариот // Биохимия, 2008: 73(3), 426 – 433.
  26. Генинг Л.В., Макарова А.В., Малашенко А.М., Тарантул В.З. Фальшивая нота ДНК-полимеразы йота в ансамбле защитников генома млекопитающих // Биохимия, 2006: 71(2), 155 – 159

Grants (Principal Investigator)

  1. RFBR 12-04-01697-а. 2012-2014.
  2.  RFBR 15-04-08398-а. 2015-2017.
  3.  RFBR and The Government of Moscow 15-34-70002-мол_а_мос, 2016-2017.
  4.  Program of The Presidium of RAS «Molecular and Cellular Biology. New groups», 2015-2018.
  5.  RFBR 17-00-00264. 2018-2020 (together with D. Zharkov and A. Rizvanov).
  6.  RSF 18-14-00354, 2018-2020.