Dolotov Oleg | Institute of Molecular Genetics
Academic degree:
Doctor of Philosophy (Biology)

Academic title:
without academic rank


Division of IMG:
Laboratory of molecular genetics of somatic cells

Position:
Senior Researcher


Telephone:
+7-499-196-02-11

Е-mail:
dolotov@img.ras.ru



Main research interests

The research interests are focused on the neuroprotective, cognitive and behavioral effects N-terminal fragments of adrenocorticotropic hormone (ACTH) and their synthetic analogs (melanocortins). The goal of the research is to understand the biological role of these peptides, mechanisms that underlie their effects and potential for the treatment of stress- and inflammation-related disorders of the central nervous system. During the course of our studies, we found that melanocortins are able to stimulate expression of neurotrophic factors in the rat brain. In the chronic unpredictable stress model we revealed the anti-stress effect of N-terminal fragments of ACTH and some their analogs such as a clinically applied ACTH(4-10) analog Semax.



Publications

  1. Grivennikov I.A., Dolotov O.V., Gol'dina Iu.I. Peptide factors in processes of proliferation, differentiation, and extended viability of mammalian nervous system cells. Mol Biol (Mosk). 1999, 33, 1, 120-126.
  2. Shadrina M.I., Dolotov O.V., Grivennikov I.A., Inozemtseva L.S., Andreeva L.A., Slominsky P.A., Limborska S.A., Myasoedov N.F. Rapid induction of neurotrophin mRNAs in rat glial cell cultures by Semax, an adrenocorticotropic hormone analog. Neurosci. Lett. 2001, 308, 115-118.
  3. Dolotov O.V., Seredenina T.S., Levitskaya N.G., Kamensky A.A., Andreeva L.A., Alfeeva L.Y., Nagaev I.Y., Zolotarev Y.A., Grivennikov I.A., Engele Y., Myasoedov N.F. The heptapeptide SEMAX stimulates BDNF expression in different areas of the rat brain in vivo. Dokl. Biol. Sci. 2003, 391, 292-295.
  4. Dolotov O.V., Zolotarev Iu.A., Dorokhova E.M., Andreeva L.A., Alfeeva L.Iu., Grivennikov I.A., Miasoedov N.F. The binding of Semax, ACTH 4-10 heptapeptide, to plasma membranes of the rat forebrain basal nuclei and its biodegradation. Bioorg. Khim. 2004, 30, 3, 241-246.
  5. Novosadova E.V., Manuilova E.S., Arsen'eva E.L., Khaidarova N.V, Dolotov O.V., Inozemtseva L.S., Kozachenkov K.Y., Tarantul V.Z., Grivennikov I.A. Different effects of enhanced and reduced expression of pub gene on the formation of embryoid bodies by cultured embryonic mouse stem cell. Bull. Exp. Biol. Med. 2005, 140, 1, 153-158.
  6. Zolotarev Iu.A., Dadaian A.K., Dolotov O.V. et al. Evenly tritium-labeled peptides and their in vivo and in vitro biodegradation. Bioorg. Khim. 2006, 32, 2, 183-191.
  7. Kovalev G.I., Abaimov D.A., Voronin M.V., Firstova J.Yu., Dolotov O.V. The study of auto- and heteroreceptor components of presynaptic dopamine re-uptake modulation in the mechanism of novel antiparkinsonian drug hemantane action in vitro. Neurochem. J., 2007, 1, 3, 253-259.
  8. Ashmarin I.P., Samonina G.E.,. Lyapina L.A, Kamenskii A.A., Levitskaya N.G., Grivennikov I.A., Dolotov O.V., Andreeva L.A., Myasoedov N.F. Natural and hybrid («chimeric») stable regulatory gliproline peptides. Pathophysiology. 2005, 11, 4, 179-185.
  9. Dolotov O.V., Karpenko E.A., Seredenina T.S., Inozemtseva L.S., Levitskaya N.G., Zolotarev Yu.A., Kamensky A.A., Grivennikov I.A., Engele J., Myasoedov N.F. Semax, an analogue of adrenocorticotropin (4–10), binds specifically and increases levels of brain-derived neurotrophic factor protein in rat basal forebrain. J. of Neurochem. 2006, 97, s1, 82-86.
  10. Zolotarev Yu.A., Dolotov O.V., Inozemtseva L.S., Dadayan A.K., Dorokhova E.M., Andreeva L.A., Alfeeva L.Yu., Grivennikov I.A., Myasoedov N.F. Degradation of the ACTH(4-10) analog Semax in the presence of rat basal forebrain cell cultures and plasma membranes. Amino Acids. 2006, 30, 4, 403-408.
  11. Dolotov O.V., Karpenko E.A., Inozemtseva L.S., Seredenina T.S., Levitskaya N.G., Rozyczka J., Dubynina E.V., Novosadova E.V., Andreeva L.A., Alfeeva L.Y., Kamensky A.A., Grivennikov I.A., Myasoedov N.F., Engele J. Semax, an analog of ACTH(4-10) with cognitive effects, regulates BDNF and trkB expression in the rat hippocampus. Brain Research. 2006, 1117, 1, 54-60.
  12. Inozemtseva L.S., Karpenko E.A., Dolotov O.V. Levitskaya N.G., Kamensky A.A., Andreeva L.A., Grivennikov I.A. Intranasal administration of the peptide Selank regulates BDNF expression in the rat hippocampus in vivo. Dokl. Biol. Sci. 2008, 421, 241-243.
  13. Grivennikov I.A., Dolotov O.V., Zolotarev Y.A., Andreeva L.A., Myasoedov N.F., Leacher L., Black I.B., Dreyfus C.F. Effects of behaviorally active ACTH (4–10) analogue – Semax on rat basal forebrain cholinergic neurons. Restorative neurology and neuroscience. 2008, 26, 1, 35-43.
  14. Dubynina E.V., Dolotov O.V The CREB Transcription Factor and Processes of Memory Formation. Neurochem. J. 2009, 3, 3, 155-163.
  15. Dubynina E.V., Inozemtseva L.S., Markov D.D., Yatsenko K.A., Dolotov O.V., Grivennikov I.A. Neurochem. J. 2009, 3, 4, 267-271.
  16. Firstova Iu.Iu., Dolotov O.V., Kondrakhin E.A., Dubynina E.V., Grivennikov I.A., Kovalev G.I. Effects of nootropic drugs on hippocampal and cortical BDNF levels in mice with different exploratory behavior efficacy. Eksp. Klin. Farmakol. 2009, 72, 6, 3-6.
  17. Glazova N.Y., Atanov M.S., Pyzgareva A.V., Andreeva L.A., Manchenko D.M., Markov D.D., Inozemtseva L.S., Dolotov O.V., Levitskaya N.G., Kamensky A.A., Grivennikov I.A., Myasoedov N.F. Neurotropic activity of ACTH(7-10)PGP, an analog of an ACTH fragment. Dokl. Biol. Sci. 2011, 440, 270-274.
  18. Volodina M.A., Sebentsova E.A., Glazova N.Y., Manchenko D.M. Inozemtseva L.S., Dolotov O.V., Andreeva L.A., Levitskaya N.G., Kamensky A.A., Myasoedov N.F. Correction of long-lasting negative effects of neonatal isolation in white rats using semax. Acta Naturae. 2012, 4, 1, 86-92.
  19. Yatsenko K.A., Glazova N.Y., Inozemtseva L.S., Andreeva L.A., Kamensky A.A., Grivennikov I.A., Levitskaya N.G., Dolotov O.V., Myasoedov N.F. Heptapeptide semax attenuates the effects of chronic unpredictable stress in rats. Dokl. Biol. Sci. 2013, 453, 353-357.
  20. Dolotov O.V., Eremin K.O., Andreeva L.A., Novosadova E.V., Raevskii K.S, Myasoedov N.F, Grivennikov I.A. Semax prevents the death of tyrosine hydroxylase-positive neurons in a mixed neuroglial cell culture derived from the embryonic rat mesencephalon in a model of 6-hydroxydopamine-induced neurotoxicity Neurochem. J. 2015, 9, 4, 295-298. doi: 10.1134/S1819712415040066
  21. Antonov S.A., Manuilova E.S., Dolotov O.V., Kobylyansky A.G., Safina D.R., Grivennikov I.A. Effect of Nerve Growth Factor on Neural Differentiation of Mouse Embryonic Stem Cells. Bull Exp Biol Med. 2017, 162, 5, 679-683. doi: 10.1007/s10517-017-3686-1
  22. Markov D.D., Yatsenko K.A., Inozemtseva L.S., Grivennikov I.A., Myasoedov N.F., Dolotov O.V. Systemic N-terminal fragments of adrenocorticotropin reduce inflammation- and stress-induced anhedonia in rats. Psychoneuroendocrinology. 2017, 82, 173-186. doi: 10.1016/j.psyneuen.2017.04.019
  23. Sukhanova Yu.A., Volodina M.A., Sebentsova E.A., Glazova N.Yu, Manchenko D.M., Inozemtseva L.S., Andreeva L.A., Dolotov O.V., Levitskaya N.G. Long-term changes in behavior and the content of BDNF in the rat brain caused by neonatal isolation: the effects of an analog of ACTH(4-10) Semax. Neurochem J. 2018, 12, 1, 53-63. doi: 10.1134/S1819712418010154
  24. Sukhanova I.A., Sebentsova E.A., Khukhareva D.D., Manchenko D.M., Glazova N.Y., Vishnyakova P.A., Inozemtseva L.S., Dolotov O.V., Vysokikh M.Y., Levitskaya N.G. Gender-dependent changes in physical development, BDNF content and GSH redox system in a model of acute neonatal hypoxia in rats. Behav Brain Res. 2018, 350, 87-98. doi: 10.1016/j.bbr.2018.05.008