Laboratory of Mechanisms of Replication of damaged DNA | Institute of Molecular Genetics

Makarova Alena Vladimirovna


List of staff

Makarova Alena Vladimirovna, Ph.D., head of laboratory
Boldinova Elizaveta, PhD student
Shilkin Evgeniy, PhD student
Gagarinskaya Diana, PhD student
Poltorachenko Valentin A., senior assistant
Makarova Anna V., technician



Main directions of research

Fidelity of replication and mutagenesis by human specialized DNA polymerases (Pol iota, Pol eta, Pol zeta, PrimPol), translesion DNA synthesis.
Studies are supported by:
  1. RFBR 17-00-00264-комфи. 2018-2020 (together with D. Zharkov and A. Rizvanov)
  2. RSF 18-14-00354, 2018-2020
  3. RFBR 19-34-90147, 2019-2021 (grant for PhD students)
  4. RFBR 18-04-00777-а, 2018-2020
  5. RFBR 18-54-00024-Бел-а, 2018-2019
Completed Grant Projects:
  1. RFBR 12-04-01697-а. 2012-2014.
  2. RFBR 15-04-08398-а. 2015-2017.
  3. RFBR and The Government of Moscow 15-34-70002-мол_а_мос, 2016-2017.
  4. Program of The Presidium of RAS «Molecular and Cellular Biology. New groups», 2015-2018.

Main scientific results

Makarova A.V.
1. Award L`oreal Unesco "For Women in Science", 2017
2. Fellowship of the President of Russian Federation, 2016-2017
3. Fellowship of Dmitry Zimin Foundation "Dynasty", 2014-2016
Boldinova E.O.
Fellowship of the President of Russian Federation, 2018-2019



  1. Yudkina A.V., Shilkin E.S., Endutkin A.V., Makarova A.V.*, Zharkov D.O.* Reading and misreading 8-oxoguanine, a paradigmatic ambiguous nucleobase // Crystals, 2019, 9(5): 269, *corresponding authors
  2. Kim D.V., Makarova A.V., Miftakhova R.R. Zharkov D.O. Base excision DNA repair deficient cells: from disease models to genotoxicity sensors // Curr Pharm Design, 2019, 25(3): 298 — 312
  3. Boldinova E.O., Khairullin R.K., Makarova A.V.*, Zharkov D.O.* Isoforms of base excision repair enzymes produced by alternative splicing // Int J Mol Sc., 2019, 20(13). pii: E3279. *corresponding authors
  4. Gagarinskaya D.I., Makarova A.V. A multifunctional protein PolDIP2 in DNA translesion synthesis // Adv Exp Med Biol, 2019, in press
  5. Boldinova E.O., Ignatov A.V., Kulbachinskiy A.V., Makarova A.V. The active site residues Gln55 and Arg73 play a key role in DNA damage bypass by S. cerevisiae Pol η // Scientific Reports, 2018, 8 (1), 1031
  6. Makarova A.V.*, Boldinova E.O., Belousova E.A., Lavrik O.I.* In vitro lesion bypass by human PrimPol // DNA Repair, 2018, 70:18-24
  7. Kazachenko K.Y., Miropolskaya N.A., Gening L.G., Tarantul V.Z., Makarova A.V. Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro // DNA Repair, 2017, 50:77-82
  8. Boldinova E.O., Wanrooij P.H., Shilkin E.S., Wanrooij S., Makarova A.V. DNA damage tolerance by eukaryotic DNA polymerase and primase PrimPol // Int. J. Mol. Sci. 2017, 18(7), pii: E1584
  9. Miropolskaya N., Petushkov I., Kulbachinskiy A., Makarova A.V. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι // Scientific Reports 2017, 7, 10194
  10. Boldinova E.O., Stojkovič G., Khairullin R., Wanrooij S., Makarova A.V. Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol // PLoS One, 2017, 12(9): e0184489.
  11. Ignatov A.V., Bondarenko K.A., Makarova A.V*. Non-bulky Lesions in Human DNA: the Ways of Formation, Repair, and Replication // Acta Naturae 2017, 9:12-26
  12. Stojkovič G.*, Makarova A.V*., Wanrooij P.H., Forslund J., Burgers P.M., Wanrooij S. Oxidative DNA damage stalls the human mitochondrial replisome // Scientific Reports, 2016: 6, 28942. * equal contribution
  13. Makarova A.V. and Burgers P.M. Eukaryotic DNA polymerase zeta // DNA Repair, 2015: 29, 47-55
  14. Makarova A.V.*, Ignatov A., Miropolskaya N, Kulbachinskiy A.* Roles of the active site residues and metal cofactors in noncanonical base-pairing during catalysis by human DNA polymerase iota // DNA Repair, 2014, 22: 67-76. *corresponding authors