Laboratory of Biochemical Genetics of Animals | Institute of Molecular Genetics

Gvozdev Vladimir Alekseevich


List of staff

Gvozdev V.A. , Doctor  of Biological Sciences, Academician of the Russian Academy of Sciences
Kogan G.L., Senior Research Scientist, Ph.D. 
Lavrov S.A., Senior Research Scientist, Ph.D. 
Mikhaleva E.A., Research Scientist, Ph.D. 
Olenina L.V., Senior Research Scientist, Ph.D.
Sokolova O.A., Research Scientist, Ph.D.
Klenov M.S., Senior Research Scientist, Ph.D. 
Riazansky S.S., Research Scientist, Ph.D.
Leinsoo T. A., Ph.D. 
Stolyarenko A.D., Research Scientist
Kotov A.A., Research Scientist, Ph.D.
Veselaya Yu.Ya., engineer
Perchikova L.V., technician
Solodovnikov A.A., student
Blokh R.S., student
Pirogov S.A., student
Adashev V.E., student


Main directions of research

Studies of gene expression regulation using Drosophila melanogaster as a model:
  1. Studies of small piRNAs in transposon repression.
  2. Investigation of the role of the microenvironment of somatic cells in the differentiation of germ cells.
  3. Analysis of cis and trans-effects of heterochromatin on target genes. 
  4. Studies of functional role of testis specific NAC protein associated to ribosomes. 

Main scientific results

A new class of small RNAs was discovered, subsequently termed piRNAs, and for the first time the role of RNAi in gene expression regulation in vivo without the use of artificial genetic constructs was demonstrated (2001).
The function of RNAi in telomere length maintenance in Drosophila and the role of a protein of the Agronaute family in the suppression of transposition was demonstrated (2005, 2006).
The repression of transposons at the level of transcription, carried out by piRNA pathways proteins was revealed (2007, 2011, 2014).
A new organelle was described – a large perinuclear granule containing piRNA pathways proteins (2009).
The role of nuclear Piwi localization in transposon repression was shown (2011).
Piwi was revealed in the nuclei of somatic and germline gonadal cells (2015).
New genes were discovered which are expressed specifically in testis germline cells and code for a subunit of the translation complex bound to the ribosome. Their amplification in the evolution of the species was traced (2005, 2012).
It was shown that Belle RNA helicase, homologous to human DBY protein the lack of which causes male sterility (Sertoli cell-only syndrome), is indispensable as a cell-autonomous factor for germline cell development and as a regulatory protein in the surrounding somatic niche cells, ensuring differentiation of germline cells and preventing their uncontrolled division accompanied by tumor-like structure formation (2016).


  1. Ilyin AA, Ryazansky SS, Doronin SA, Olenkina OM, Mikhaleva EA, Yakushev EY, Abramov YA, Belyakin SN, Ivankin AV, Pindyurin AV, Gvozdev VA, Klenov MS, Shevelyov YY. Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. Nucleic Acids Res. 2017 Jul 27;45(13):7666-7680.
  2. Kotov AA, Olenkina OM, Godneeva BK, Adashev VE, Olenina LV. Progress in understanding the molecular functions of DDX3Y (DBY) in male germ cell development and maintenance. Biosci Trends. 2017 Mar 22;11(1):46-53.
  3. Ryazansky SS, Stolyarenko AD, Klenov MS, Gvozdev VA. Induction of Transposon Silencing in the Drosophila Germline. Biochemistry (Mosc). 2017 May;82(5):565-571.
  4. Ryazansky SS, Kotov AA, Kibanov MV, Akulenko NV, Korbut AP, Lavrov SA, Gvozdev VA, Olenina LV. RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila. Eur J Cell Biol. 2016 Sep;95(9):311-22.
  5. Kotov AA, Olenkina OM, Kibanov MV, Olenina LV. RNA helicase Belle (DDX3) is essential for male germline stem cell maintenance and division in Drosophila. Biochim Biophys Acta. 2016 Jun;1863(6 Pt A):1093-105.
  6. Abramov YA, Shatskikh AS, Maksimenko OG, Bonaccorsi S, Gvozdev VA, Lavrov SA. The Differences Between Cis- and Trans-Gene Inactivation Caused by Heterochromatin in Drosophila. Genetics. 2016 Jan;202(1):93-106.
  7. Mikhaleva EA, Yakushev EIu, Stolyarenko AD, Klenov MS, Pozovskiĭ IaM, Gvozdev VA. PIWI protein as a nucleolus visitor in Drosophila melanogaster. Mol Biol (Mosk). 2015. Jan-Feb;49(1):184-9.
  8. Klenov MS, Lavrov SA, Korbut AV, Stolyarenko AD, Yakushev, EYu, Reuter M,  Pillai R, Gvozdev VA. Impact of nuclear Piwi elimination on chromatin state in Drosophila melanogaster ovaries. Nucl Acids Res. 2014. 42 N 10:6208-6218.
  9. Kibanov MV, Kotov AA, Olenina LV. Multicolor fluorescence imaging of whole-mount Drosophila testes for studying spermatogenesis. Anal Biochem. 2013. 436(1):55-64.
  10. Оlenkina O.M., Egorova K. S., Kibanov M.V., Gervaziev Y.V., Gvozdev V.A., Olenina L.V. Promoter contribution of the testis-specific expression of Stellate gene family in Drosophila melanogaster. Gene, 2012. 499: 143-153.
  11. Kogan G.L., Usakin L.A., Ryazansky S.S., Gvozdev V.A. Expansion and Evolution of the X-linked testis specific multigene families in the melanogaster species subgroup. PLoS One 2012. 7, e37738.
  12. Kibanov MV, Gvozdev VA, Olenina LV. Germ granules in spermatogenesis of Drosophila: Evidences of contribution to the piRNA silencing. Communicative & Integrative Biology, 2012. 5:130-133.
  13. Kibanov M.V., Egorova K.S., Ryazansky S.S.,Sokolova O.A., Kotov A.A., Olenkina O.M., Stolyarenko A.D., Gvozdev V.A. , Olenina L.V. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Molecular Biology of the Cell, 2011. 22, 3410-3419.
  14. Ryazansky S.S., Gvozdev V.A., Berezikov E. Evidence of post-transcriptional regulation of clustered microRNAs in Drosophila. BMC Genomics, 2011. 12: 371.
  15. Klenov M.S., Sokolova O.A., Yakushev E.Yu,, Stolyarenko A.D., Mikhaleva E.A., Lavrov S.A., Gvozdev V.A. Separation of stem cell maintenance and transposon silencing functions of the Piwi protein. Proc. Natl. Acad. Sci. 2011. 108: 18760-18765.
  16. Egorova KS, Olenkina OM, Kibanov MV, Kalmykova AI, Gvozdev VA, Olenina LV.  Genetically derepressed nucleoplasmic Stellate protein in spermatocytes of D.melanogaster interacts with the catalytic subunit of protein kinase 2 and carries histone-like methylated mark. J Mol Biol. 2009. 389(5):895-906.
  17. Kotelnikov RN, Klenov MS, Rozovsky YM, Olenina LV, Kibanov MV, Gvozdev VA. Peculiarities of RNA-mediated post-transcriptional silencing of Stellate repeats in testes of Drosophila melanogaster. Nucleic Acids Res. 2009 Jun;37(10):3254-63.
  18. Klenov MS, Lavrov SA, Stolyarenko AD, Ryazansky SS, Aravin AA, Tuschl T, Gvozdev VA. Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline. Nucl Acids Res, 2007, 35:5430-5438.
  19. Usakin L.,  Abad J., Vagin V.V.,  de Pablos B,  Villasante A.,  Gvozdev V.A.  Transcription of the 1.688 satellite DNA family is under the control of RNAi machinery in Drosophila melanogaster ovaries. Genetics, 2007, 176:1343-1349.
  20. Savitsky M., Kwon D., Georgiev P., Kalmykova A.,Gvozdev V.  Telomere elongation is under the control of the RNA-based mechanism in the Drosophila germline. Genes and Development.  2006, 20:345-354.
  21. Vagin VV, Sigova A, Li C, Seitz H, Gvozdev V, Zamore PD. A distinct small RNA pathway silences selfish genetic elements in the germline. Science. 2006. Jul 21;313(5785):320-4.
  22. Kalmykova AI, Klenov MS, Gvozdev VA. Argonaute protein PIWI controls mobilization of retrotransposons in the Drosophila male germline.  Nucleic Acids Res 2005, 33: 2052-2059.
  23. Usakin LA, Kogan GL, Kalmykova AI, Gvozdev VA. An alien promoter capture as a primary step of the evolution of testes expressed repeats in Drosophila melanogaster genome. Mol.Biol. Evol., 2005, 22:1555-1560.
  24. Abramov YA, Kogan GL, Tolchkov EV, Rasheva VI, Lavrov SA, Bonaccorsi S, Kramerova IA, Gvozdev VA. Eu-heterochromatic rearrangements induce replication of heterochromatic sequences normally underreplicated in polytene chromosomes of Drosophila melanogaster. Genetics 2005, 171:1673-1681.
  25. Gvozdev VA, Kogan GL, Usakin LA. Y chromosome as a target of acquired and amplified genetic material in the course of evolution.  BioEssays, 2005, 27:1256-1262.
  26. Aravin A.A., Klenov M.S., Vagin V.V.,Bantignies F., Cavalli G., Gvozdev V.A. Dissection of a natural RNA silencing process in the Drosophila melanogaster germline Mol Cell Biol., 2004, 24, 6742-6750.
  27. Vagin V.V., Klenov M S., Kalmykova A.I, Stolyarenko A.D., Kotelnikov R  Gvozdev V.A. The RNA interference proteins and vasa locus are involved in the silencing of retrotransposons in the female germline of Drosophila melanogaster. RNA Biology, 2004, v.1, 54-58.
  28. Kalmykova A.I., Kwon D.A., Rosovsky Ya.M.,Hueber N., Capy P.,Maisonhaute C.,Gvozdev V.A.  Selective expansion of the newly evolved genomic variants of retrotransposon 1731 in the Drosophila genomes. Mol. Biol. Evol. 2004, v.21, 2281-2289.
  29. G.L. Kogan, A.V. Tulin, A.A. Aravin, Yu. A. Abramov, A. I. Kalmykova, C. Maisonhaute, V. A. Gvozdev. GATE retrotransposon in Drosophila melanogaster: mobility in heterochromatin and peculiarities of expression in germinal tissues. Mol. Gen.Genom. 2003, 269: 234-242.
  30. Kalmykova AI, Shevelyov YY, Polesskaya OO, Dobritsa AA, Evstafieva AG, Boldyreff B, Issinger O-G, Gvozdev VA. CK2βtes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster. Eur. J. Biochem., 2002, 269:1418-1427.
  31. Aravin A.A., Naumova N.M., Tulin A.A., Vagin V.V., Rozovsky Y.M., Gvozdev V.A.. Double stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D.melanogaster genome. Curr. Biol., 2001, v. 11, p.1017-1027.
  32. Tolchkov E.V., Rasheva V.I., Bonaccorsi S., Westphal T., V.A.Gvozdev. The size and internal structure of a heterochromatic block determine its ability to induce position effect variegation in Drosophila melanogaster. Genetics, 2000. 154, p. 1611-1626.
  33. Kogan GL, Epstein VN, Aravin AA, Gvozdev VA. Molecular Evolution of Two Paralogous Tandemly Repeated Heterochromatic Gene Clusters Linked to the X and Y Chromosomes of Drosophila melanogaster. Mol Biol Evol. 2000. v. 17, p. 697-702.