Group "Specialized DNA Polymerases" | Institute of Molecular Genetics

Makarova Alena Vladimirovna


List of staff

Makarova Alena Vladimirovna, Ph.D., Head. group
Kozyreva Ksenia Aleksandrovna, graduate student
Boldinova Elizaveta Olegovna, student


Main directions of research

Fidelity of replication and mutagenesis by human specialized DNA polymerases (Pol iota, Pol eta, Pol zeta, PrimPol), translesion DNA synthesis. Studies were supported by:
  1. RFBR, 12-04-01697-а. «Molecular mechanisms of human DNA polymerase iota functioning during replication and repair», 2012-2014.
  2. Dmitry Zimin foundation “Dynasty”, Postdoc fellowship «Effect of DNA lesions on efficiency and fidelity of human Y- and B-family DNA polymerases», 2014-2016.
  3. Program of Presidium of RAS «Molecular and Cellular Biology» New groups, «Replication mechanisms by human specialized DNA polymerases on damaged and nondamaged DNA», 2015-2017.
  4. RFBR, 15-04-08398-а. «Ribonucleotide incorporation by human specialized DNA polymerases on undamaged and damaged DNA», 2015-2017.
  5. RFBR,15-34-70002-мос-а. «Effect of clinical mutations of human specialized DNA polymerases on DNA replication», 2016-2017.


  1. Makarova A.V. and Burgers P.M. Eukaryotic DNA polymerase zeta // DNA Repair, 2015: 29, 47-55. (review)
  2. Makarova A.V., Ignatov A., Miropolskaya N, Kulbachinskiy A. Roles of the active site residues and metal cofactors in noncanonical base-pairing during catalysis by human DNA polymerase iota // DNA Repair, 2014, 22: 67-76.
  3. Makarova A.V. and Burgers P. “DNA polymerase ζ and Mutagenesis in Eukaryotic Cells,.”, in “Translesion DNA polymerases: from DNA repair and beyond”, Maiorano D. and Hoffmann J-S., Eds.: Research Signpost, 2015, 153-177. (chapter in a book).
  4. Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Error-prone Replication Bypass of the Primary Aflatoxin B1 DNA Adduct, AFB1-N7-Gua // J Biol Chem, 2014: 289(26), 18497-18506.
  5. Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Molecular basis of aflatoxin-induced mutagenesis – role of the aflatoxin B1-formamidopyrimidine adduct // Carcinogenesis, 2014: 35(7), 1461-1468.
  6. Makarova A.V., Nick McElhinny S.A., Watts B.E., Kunkel T.A., Burgers P.M. Ribonucleotide incorporation by yeast DNA polymerase ζ // DNA Repair, 2014: 18, 63-67.
  7. Makarova A.V., Stodola J.L., Burgers P.M. A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis // Nucleic Acids Research, 2012: 40(22), 11618 – 11626.
  8. Smith L.A., Makarova A.V., Samson L., Thiesen K.E., Dhar A., Bessho T. Bypass of a psoraen DNA interstrand cross-link by DNA polymerases beta, iota, and kappa in vitro // Biochemistry, 2012:51, 8931 – 8938.
  9. Makarova A.V., Kulbachinskiy A.V. Structure of human DNA polymerase iota and the mechenism of DNA synthesis // Biochemistry(Mosc), 2012: 77(6), 547 – 61. (review)
  10. Lachin A.V., Kazakov A.A., Makarova A.V., Pavlov Y.I., Efremova A.S., Shram S.I., Тarantul V.Z., Gening L.V. Isolation and characterization of high affinity aptamers selected against DNA polymerase iota // Nucleic Acid Ther., 2012: 22 (1), 49 – 57.
  11. Makarova I.V., Kazakov A.A., Makarova A.V., Khaidarova N.V., Kozikova L.V., Nenasheva V.N., Gening L.V., Tarantul V.Z., Andreeva L.E. Transient expression and activity of human DNA polymerase iota in loach embryos // Biotechnology Letters, 2012: 34 (2), 205 – 212.
  12. Makarova A.V., Grabow C., Gening L.V., Tarantul V.Z., Tahirov T.T., Bessho T. Pavlov Y.I. Inaccurate DNA synthesis due to the presence of DNA polymerase iota activity in whole cell extracts // PLOS One, 2011: 6 (1), 1 – 10.
  13. Kazakov A.A., Makarova A.V., Grishina E.E., Tarantul V.Z., Gening L.V. Activity of DNA polymerase iota in human basal cell carcinoma // Curr. Topics Biochem. Res, 2010: 12 (2), 39 – 49.
  14. Makarova A.V., Gening L.V., Makarova I.V., Tarantul V.Z. Activity of error-prone DNA polymerase iota in different periods of house mouse Mus musculus ontogeny.  Ontogenez, 2008, 39(5):367-73.
  15. Makarova A.V., Tarantul V.Z., Geninng L.V. Evolution of DNA polymerase iota structure and functions in eukaryotes // Biochemistry (Mosc), 2008 (3):346-52.
  16. Gening L.V., Makarova A.V., Malashenko A.M., Tarantul V.Z. A false note of DNA polymerase iota in the choir of genome caretakers in mammals // Biochemistry (Mosc). 2006, 71(2):155-9.