Savitskaya Ekaterina Evgenievna | Institute of Molecular Genetics
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Academic degree:
Doctor of Philosophy (Biology)
Academic title:
without academic rank
Division of IMG:
Laboratory of gene expression regulation of mobile elements of prokaryotes
Position:
Senior Researcher
Telephone:
+7-499-196-18-52
Е-mail: |
Main research interests
CRISPR-Cas adaptive immune system of prokaryotes. Mechanisms of CRISPR-adaptation and CRISPR-interference.
Principal advances of the group:
- On the basisi of high throughput data analysis of new spacers it was shown that in the course of primed adaptation new spacers come from the DNA strand of primed priming protospacer (Savitskaya et al., 2013).
- When a protospacer containes a spacer acquisition motif AAG, spacer orientation that generates functional protective crRNA is strongly preferred. All other protospacers give rise to spacers oriented in both ways at comparable frequencie. (Shmakov et al., 2014).
- CRISPR primed adaptation based procedure was elaborated to very fast and rapid construction of multiple E.coli strains capable of targeting any phage or plasmid of interest (Strotskaya et al., 2015).
Principal advances of the group:
- On the basisi of high throughput data analysis of new spacers it was shown that in the course of primed adaptation new spacers come from the DNA strand of primed priming protospacer (Savitskaya et al., 2013).
- When a protospacer containes a spacer acquisition motif AAG, spacer orientation that generates functional protective crRNA is strongly preferred. All other protospacers give rise to spacers oriented in both ways at comparable frequencie. (Shmakov et al., 2014).
- CRISPR primed adaptation based procedure was elaborated to very fast and rapid construction of multiple E.coli strains capable of targeting any phage or plasmid of interest (Strotskaya et al., 2015).
Publications
- The Cas6e ribonuclease is not required for interference and adaptation by the E. coli type I-E CRISPR-Cas system. Semenova E, Kuznedelov K, Datsenko KA, Boudry PM, Savitskaya E, Medvedeva S, Beloglazova N, Logacheva M, Yakunin AF, Severinov K. Nucleic Acids Res. 2015 Jul 13;43(12):6049-61.
- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR. Strotskaya A, Semenova E, Savitskaya E, Severinov K. Methods Mol Biol. 2015;1311:147-59.
- The Digital Ageing Atlas: integrating the diversity of age-related changes into a unified resource. Craig T, Smelick C, Tacutu R, Wuttke D, Wood SH, Stanley H, Janssens G, Savitskaya E, Moskalev A, Arking R, de Magalhães JP. Nucleic Acids Res. 2015;43(Database issue):D873-8.
- Pervasive Generation of Oppositely-Oriented Spacers during CRISPR Adaptation. Shmakov S*, Savitskaya E*, Semenova E, Datsenko K, Severinov K. Nucleic Acids Res. 2014;42(9):5907-16. *-equally contributed
- High-throughput analysis of type I-E CRISPR/Cas spacer acquisition in E. coli. Savitskaya E, Semenova E, Dedkov V, Metlitskaya A, Severinov K. RNA Biol. 2013 Apr 25;10(5).
- Interaction between a pair of gypsy insulators or between heterologous gypsy and Wari insulators modulates Flp site-specific recombination in Drosophila melanogaster. Krivega M, Savitskaya E, Krivega I, Karakozova M, Parshikov A, Golovnin A, Georgiev P. Chromosoma. 2010 Aug;119(4):425-34.
- Several copies of insulator from MDG4 can determine the interaction between positively and negatively acting regulatory elements and promoter of the miniwhite gene of Drosophila melanogaster. Kostiuchenko MV, Savitskaia EE, Krivega MN, Georgiev PG. Genetika. 2008 Dec;44(12):1693-7. Russian.
- Kostyuchenko M, Savitskaya E, Koryagina E, Melnikova L, Karakozova M, Georgiev P Zeste can facilitate long-range enhancer-promoter communication and insulator bypass in Drosophila melanogaster. Chromosoma. 2009 Oct;118(5):665-74.
- Ruzov A*, Savitskaya E*, Hackett JA, Reddington JP, Prokhortchouk A, Madej MJ, Chekanov N, Li M, Dunican DS, Prokhortchouk E, Pennings S, Meehan RR. The non-methylated DNA-binding function of Kaiso is not required in early Xenopus laevis development. Development. 2009 Mar;136(5):729-38. *-equally contributed
- Kostyuchenko MV, Savitskaya EE, Volkov IA, Golovnin AK, Georgiev PG. Study of functional interaction between three copies of the insulator from the MDG4 transposable element in the model system of the miniwhite gene of the Drosophila melanogaster. Dokl Biochem Biophys. 2008 Jul-Aug;421:239-43. Russian.
- Chetverina D, Savitskaya E, Maksimenko O, Melnikova L, Zaytseva O, Parshikov A, Galkin AV, Georgiev P. Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs. Nucleic Acids Res. 2008 Feb;36(3):929-37.
- Comet I, Savitskaya E, Schuettengruber B, Negre N, Lavrov S, Parshikov A, Juge F, Gracheva E, Georgiev P, Cavalli G. PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter. Dev Cell. 2006 Jul;11(1):117-24.
- Kostyuchenko MV, Savitskaya EE, Karakozova MV, Georgiev PG. Study of the regulatory region of gene white of Drosophila melanogaster. Dokl Biochem Biophys. 2005 Nov-Dec;405:383-7. Russian.
- Savitskaya E, Melnikova L, Kostuchenko M, Kravchenko E, Pomerantseva E, Boikova T, Chetverina D, Parshikov A, Zobacheva P, Gracheva E, A, Georgiev P. Study of long-distance functional interactions between Su(Hw) insulators that can regulate enhancer-promoter communication in Drosophila melanogaster. Mol Cell Biol. 2006 Feb;26(3):754-61.
- Pomerantseva E, Biryukova I, Silicheva R, Savitskaya E, Golovnin A, Georgiev P. Transposition of Regulatory Elements by P-Element-Mediated Rearrangements in Drosophila melanogaster.Genetics. 2006 Apr;172(4):2283-91. Epub 2005 Dec 30.
- Kravchenko E, Savitskaya E, Kravchuk O, Parshikov A, Georgiev P, Savitsky M. Pairing between gypsy insulators facilitates the enhancer action in trans throughout the Drosophila genome. Mol Cell Biol. 2005 Nov;25(21):9283-91.
- Kostyuchenko MV, Georgiev PG, Savitskaya EE. The promoter region of the yellow gene of Drosophila melanogaster contains excess regulatory elements.Dokl Biochem Biophys. 2004 Nov-Dec;399:374-5. Russian.
- Karakozova M, Savitskaya E, Melnikova L, Parshikov A, Georgiev P. The Mod(mdg4) component of the Su(Hw) insulator inserted in the P transposon can repress its mobility in Drosophila melanogaster.Genetics. 2004 Jul;167(3):1275-80.
- Karakozova MV, Savitskaya EE, Parshikov AF, Georgiev PG. Cis-modifiers of the yellow gene repression induced by the Su(Hw) insulator in the absence of the Mod(mdg4) protein in Drosophila melanogaster. Dokl Biochem Biophys. 2003 Mar-Apr;389:110-3. Russian.
- Golovnin A, Birukova I, Romanova O, Silicheva M, Parshikov A, Savitskaya E, Pirrotta V, Georgiev P. An endogenous Su(Hw) insulator separates the yellow gene from the Achaete-scute gene complex in Drosophila. Development. 2003 Jul;130(14):3249-58.
- Muravyova E**, Golovnin A, Gracheva E, Parshikov A, Belenkaya T, Pirrotta V, Georgiev P. Loss of insulator activity by paired Su(Hw) chromatin insulators. Science. 2001 Jan 19;291(5503):495-8.
- Murav'eva EE*, Golovnin AK, Parshikov AF, Georgiev PG. New properties of the Su(Hw) insulator. Dokl Biochem Biophys. 2001 May-Jun;378:181-5. Russian.
- Georgiev PG, Murav'eva EE*, Golovnin AK, Gracheva EM, Belen'kaia TIu. [Insulators and interaction between long-distance regulatory elements in higher eukaryotes] Genetika. 2000 Dec;36(12):1588-97. Review. Russian.