Safina Dina Rashidovna | Institute of Molecular Genetics

Academic degree:
Doctor of Philosophy (Chemistry)

Academic title:
without academic rank

Division of IMG:
Laboratory of protein engineering




Main research interests

Research of structure-functional relationships in proteins.
Studying of a role of pro-sequence in formation of spatial structures and functioning of molecules of neurotrophin; effect of neurotrophin and proneurotrophin on regulation of calcium homeostasis of neurons. It is for the first time shown that recombinant human proNT-3 has impact on a calcium homeostasis of neurons of a brain.
Research of mechanisms of an expression of foreign DNA in eukaryotic systems. Creation of new vectors for an effective expression of target genes in eukaryotic systems.

Teaching activities

2008 - present: the assistant, department of biotechnology and industrial pharmacy faculty of Biotechnologies of organic synthesis of MITHT of M. V. Lomonosov.


  1. D.R. Safina, A.M. Surin, V.G. Pinelis, S.V. Kostrov. Effect of neurotrophin-3 precursor on glutamate-induced calcium homeostasis deregulation in rat cerebellum granule cells. // J Neurosci Res. 2015 Sep 8. doi: 10.1002/jnr.23667. [Epub ahead of print].
  2. S.A. Antonov, E.S. Manuilova, O.V. Dolotov, A.G. Kobylyansky, S.V. Kostrov, D.R. Safina, N.V. Haydarova, I.A. Grivennikov. [Derivation of mouse embryonic stem cells with inducible expression of human nerve growth factor]. // Yu.A. Ovchinnikov bulletin of biotechnology and physical and chemical biology. 2012. V.8. №3. P.5-12. Russian.
  3. D. Safina, L. Rafieva, I. Demidyuk, E. Gasanov, G. Chestukhina, S. Kostrov. Involvement of propeptides in formation of catalytically active metalloproteinase from Thermoactinomyces sp. // Protein & Peptide Letters. 2011. V.18. №11. P. 1119-1125.
  4. Rafieva L.M., Safina D.R., Demidyuk I.V., Kostrov S.V. [Production of human recombinant neurotrophins for biomedical studies]. // Review MITHT. 2011. V.6. №2. P.51-57. Russian.
  5. Safina D.R., Rafieva L.M., Koval' A.V., Shkurina E.E., Dmitrieva V.G., Raevskaia N.M., Gasanov E.V., Demidiuk I.V., Kostrov S.V. [Oligomeric organization of recombinant human neurotrophins expressed in Escherichia coli cells].. 34. №3. .327-32. .
  6. M. Sharipova, N. Balaban, A. Kayumov, Yu. Kirillova, A. Mardanova, L. Gabdrakhmanova, I. Leshchinskaya, G. Rudenskaya, T. Akimkina, D. Safina, I. Demidyuk, S. Kostrov. The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis. // Microbiological Research. 2008. V.163. №1. P.39-50.
  7. Demidyuk I., Gasanov E., Safina D., Kostrov S. Structural organization of precursors of thermolysin-like proteinases. // The Protein Journal. 2008 V.27. №6. P.343-354.
  8. Demidyuk I.V., Kalashnikov A.E., Gromova T.Yu., Gasanov E.V., Safina D.R., Zabolotskaya M.V., Rudenskaya G.N., Kostrov S.V. Cloning, sequencing, expression, and characterization of protealysin, a novel neutral proteinase from Serratia proteamaculans representing a new group of thermolysin-like proteases with short N-terminal region of precursor. // Protein Expression and Purification. 2006. V.47 №2. P.551-61.
  9. Chastukhina I.B., Sharipova M.R., Gabdrakhmanova L.A., Balaban N.P., Safina D.R., Kostrov S.V., Rudenskaia G.N., Leshchinskaia I.B. [The regulation of Bacillus intermedius glutamyl endopeptidase biosynthesis in the recombinant Bacillus subtilis strain during sporulation]. // Mikrobiologiia. 2004. V. 73. №3. P.335-42. Russian.
  10. Demidiuk I.V., Zabolotskaia M.V., Velishaeva N.S., Safina D.R., Kostrov S.V. [The pro-dependent folding of microbial proteolytic enzymes]. // Mol Gen Mikrobiol Virusol. 2003. V.4. 11-15. Russian.
  11. Demidiuk I.V., Zabolotskaia M.V., Safina D.R., Kostrov S.V. [Molecular mechanisms of stabilization of proteolytic enzymes: a model of thermolysin-like microbial metalloproteases]. // Bioorg Khim. 2003. V.29. №5. P. 461-469. Russian.