Olenkina Oxana Mikhailovna | Institute of Molecular Genetics

Academic degree:
Doctor of Philosophy (Biology)

Academic title:
without academic rank

Division of IMG:
Laboratory of analysis of gene regulation




Main research interests

I am interested in the transcriptional regulation of testis-specific genes of Drosophila at the minimal promoter level and at the chromosome level (dosage compensation and X chromosome inactivation during spermatogenesis).
2001-2005 Study of reporter genes inactivation by cloned heterochromatic Stellate repeats in Drosophila melanogaster
2005-2012 Promoter analysis of testis-specific genes Stellate: identification of cis-acting elements, E-boxes, the loss of which greatly diminished the reporter gene expression in Drosophila testes; identification of the E-box binding factor dUSF (Drosophila ortholog of mammalian USF). All three E-boxes were preserved in the promoters of both euchromatic and heterochromatic Stellate clusters. Two analogous E-boxes were detected in the promoters of 5′-copies of the duplicated βNACtes gene pairs. A new type of testis-specific core promoter was characterized and dUSF was identified as its interacting transcription factor. It was also shown that the activity of the Stellate promoter is determined by chromatin context of the X-chromosome in male germinal cells, and it increases at about twofold when relocating in autosomes.
2012-2015 Participant of studies:
  • «The study of miRNAs functions in spermatogenesis of Drosophila melanogaster»
  • «Study of the Spindle E RNA-helicase function in the piRNA silencing pathway in germinal cells of Drosophila melanogaster»
  • «The role of Vasa and Spindle E RNA-helicases in gametogenesis and genome integrity defense via piRNA silencing pathway in germinal cells of Drosophila melanogaster»
  • «Essential functions of RNA helicase Belle in the maintenance and differentiation of Drosophila male germ cells»
  • «Whole-genome study of dynamics of lamina-associated domains during spermatogenesis in Drosophila»
  • «Investigation of highly homologous testis expressed genes in D. melanogaster encoding evolutionarily conserved proteins presumably involved in responses to stress factors and the regulation of apoptotic program»


  1. Рязанский С. С., Михалева Е. А., Оленкина О. М. Существенные функции микроРНК в репродуктивных органах животных. Молекулярная биология, 2014, т. 48 (3), 371–385
  2. Olenkina O.M., Egorova K.S., Kibanov M.V., Gervaziev Y.V., Gvozdev V.A., Olenina L.V. Promoter contribution to the testis-specific expression of Stellate gene family in Drosophila melanogaster. Gene, 2012, 499(1), 143-153.
  3. О.М. Оленкина, К.С. Егорова, А.А. Аравин, Н.М. Наумова, В.А. Гвоздев, Л.В. Оленина. Картирование цис-регуляторных участков в промоторе семенник-специфичных генов Stellate у Drosophila melanogaster. Биохимия, 2012, т. 77 (11), 1536 – 1545.
  4. Ksenia S. Egorova, Oxana M. Olenkina, Ludmila V. Olenina. Significance of Stellate Genes in Spermatogenesis of Drosophila melanogaster. (Chapter in «Drosophila Melanogaster: Life Cycle, Genetics and Development», ed. M. Spindler-Barth, Nova Science publishers, 2012, p.39-61, ISBN: 978-1-61470-426-3.
  5. Kibanov M.V., Egorova K.S., Ryazansky S.S., Sokolova O.A., Kotov A.A., Olenkina O.M., Stolyarenko A.D., Gvozdev V.A., Olenina L.V. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Molecular Biology of the Cell, 2011, v. 22(18), p. 3410-3419.
  6. К.С. Егорова, О.М. Оленкина и Л.В. Оленина. Метилирование негистоновых белков по остакам лизина как способ регуляции их стабильности и функциональности. Биохимия, 2010, т. 75(5), 613-628
  7. Ksenia S. Egorova, Oxana M. Olenkina, Mikhail V. Kibanov, Alla I. Kalmykova, Vladimir A. Gvozdev and Ludmila V. Olenina. Genetically derepressed nucleoplasmic Stellate protein in spermatocytes of D. melanogaster interacts with the catalytic subunit of protein kinase 2 and carries histone-like lysine-methylated mark. J. Mol Biol., 2009, v. 389(5), p.895-906.
  8. Karr J, Vagin V, Chen K, Ganesan S, Olenkina O, Gvozdev V, Featherstone DE. Regulation of glutamate receptor subunit availability by microRNAs. J Cell Biol. 2009 185:685-97.
  9. Gvozdev VA, Aravin AA, Abramov YA, Klenov MS, Kogan GL, Lavrov SA, Naumova NM, Olenkina OM, Tulin AV, Vagin VV. Stellate repeats: targets of silencing and modules causing cis-inactivation and trans-activation. Genetica, 2003; 117(2-3): 239-45
  10. Н. М. Наумова, О. М. Оленкина, В. А. Гвоздев. Инактивация репортерных генов клонированными гетерохроматиновыми повторами Drosophila melanogaster сопровождается компактизацией хроматина. Генетика, 2003, т. 39(5), 682-686.