Makarova Alena Vladimirovna | Institute of Molecular Genetics

Makarova Alena Vladimirovna

Academic degree:
Doctor of Philosophy (Biology)

Academic title:
without academic rank

Division of IMG:
Laboratory “Mechanisms of Replication of damaged DNA”

Head of Laboratory



Main research interests

Fidelity of replication and mutagenesis by human specialized DNA polymerases (Pol iota, Pol eta, Pol zeta, PrimPol), translesion DNA synthesis.

Grants (Principal Investigator)

Studies are supported by:

  1. RFBR 17-00-00264-комфи. 2018-2020 (together with D. Zharkov and A. Rizvanov)
  2. RSF 18-14-00354, 2018-2020
  3. RFBR 19-34-90147, 2019-2021 (grant for PhD students)
  4. RFBR 18-04-00777-а, 2018-2020
  5. RFBR 18-54-00024-Бел-а, 2018-2019

Completed Grant Projects:

  1. RFBR 12-04-01697-а. 2012-2014.
  2. RFBR 15-04-08398-а. 2015-2017.
  3. RFBR and The Government of Moscow 15-34-70002-мол_а_мос, 2016-2017.
  4. Program of The Presidium of RAS «Molecular and Cellular Biology. New groups», 2015-2018.

Teaching activities

Boldinova Elizaveta, PhD student.
Shilkin Evgeniy, PhD student.
Gagarinskaya Diana, PhD student.

Awards, achievements, memberships

1. Postdoctoral fellowship of D. Zimin foundation “Dynasty”, 2014-2016.
2. Fellowship of the President of Russian Federation for Young Scientists, 2016-2017.
3. The L'Oréal-UNESCO For Women in Science (national fellowship, Moscow. Russia), 2017


  1. Yudkina A.V., Shilkin E.S., Endutkin A.V., Makarova A.V.*, Zharkov D.O.* Reading and misreading 8-oxoguanine, a paradigmatic ambiguous nucleobase // Crystals, 2019, 9(5): 269, *corresponding authors
  2. Kim D.V., Makarova A.V., Miftakhova R.R. Zharkov D.O. Base excision DNA repair deficient cells: from disease models to genotoxicity sensors // Curr Pharm Design, 2019, 25(3): 298 — 312
  3. Boldinova E.O., Khairullin R.K., Makarova A.V.*, Zharkov D.O.* Isoforms of base excision repair enzymes produced by alternative splicing // Int J Mol Sc., 2019, 20(13). pii: E3279. *corresponding authors
  4. Gagarinskaya D.I., Makarova A.V. A multifunctional protein PolDIP2 in DNA translesion synthesis // Adv Exp Med Biol, 2019, in press
  5. Boldinova E.O., Ignatov A.V., Kulbachinskiy A.V., Makarova A.V. The active site residues Gln55 and Arg73 play a key role in DNA damage bypass by S. cerevisiae Pol η // Scientific Reports, 2018, 8 (1), 1031
  6. Makarova A.V.*, Boldinova E.O., Belousova E.A., Lavrik O.I.* In vitro lesion bypass by human PrimPol // DNA Repair, 2018, 70:18-24
  7. Miropolskaya N., Petushkov I., Kulbachinskiy A., Makarova A.V. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι // Scientific Reports 2017, 7, 10194
  8. Kazachenko K.Y., Miropolskaya N.A., Gening L.G., Tarantul V.Z., Makarova A.V. Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro // DNA Repair, 2017, 50:77-82.
  9. Boldinova E.O., Wanrooij P.H., Shilkin E.S., Wanrooij S.*, Makarova A.V.* DNA damage tolerance by eukaryotic DNA polymerase and primase PrimPol // Int. J. Mol. Sci. 2017, 18(7), pii: E1584. *corresponding authors
  10. Boldinova E.O., Stojkovič G., Khairullin R., Wanrooij S.*, Makarova A.V.* Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol // PLoS One, 2017, PLoS ONE 12(9): e0184489. *corresponding authors
  11. Ignatov A.V., Bondarenko K.A., Makarova A.V.  Non-bulky Lesions in Human DNA: the Ways of Formation, Repair, and Replication // Acta Naturae 2017, 9(3):12-26.
  12. Kochenova O.V., Bezalel-Buch R., Tran P., Makarova A.V., Chabes A., Burgers P.M., Shcherbakova P.V. Yeast DNA polymerase ζ maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations // Nucleic Acids Research, 2017, 45(3):1200-1218
  13. Sawicka M., Wanrooij P.H., Darbari V.C., Tannous E., Hailemariam S., Bose D., Makarova A.V., Burgers P.M., Zhang X. The dimeric architecture of checkpoint kinases Mec1/ATR and Tel1/ATM reveal a common structural organisation // J Biol Chem, 2016: 291,13436-13447.
  14. Stojkovič G.*, Makarova A.V*., Wanrooij P.H., Forslund J., Burgers P.M., Wanrooij S. Oxidative DNA damage stalls the human mitochondrial replisome // Scientific Reports, 2016: 6, 28942. * equal contribution
  15. Makarova A.V. and Burgers P.M. Eukaryotic DNA polymerase zeta // DNA Repair, 2015: 29, 47-55. (review)
  16. Makarova A.V.*, Ignatov A., Miropolskaya N, Kulbachinskiy A.* Roles of the active site residues and metal cofactors in noncanonical base-pairing during catalysis by human DNA polymerase iota // DNA Repair, 2014, 22: 67-76. *corresponding authors
  17. Makarova A.V. and Burgers P. “DNA polymerase ζ and Mutagenesis in Eukaryotic Cells,.”, in “Translesion DNA polymerases: from DNA repair and beyond”, Maiorano D. and Hoffmann J-S., Eds.: Research Signpost, 2015, 153-177. (chapter in a book).
  18. Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Error-prone Replication Bypass of the Primary Aflatoxin B1 DNA Adduct, AFB1-N7-Gua // J Biol Chem, 2014: 289(26), 18497-18506.
  19. Lin Y.C., Li L., Makarova A.V., Burgers P.M., Stone M.P., Lloyd R.S. Molecular basis of aflatoxin-induced mutagenesis – role of the aflatoxin B1-formamidopyrimidine adduct // Carcinogenesis, 2014: 35(7), 1461-1468.
  20. Makarova A.V., Nick McElhinny S.A., Watts B.E., Kunkel T.A., Burgers P.M. Ribonucleotide incorporation by yeast DNA polymerase ζ // DNA Repair, 2014: 18, 63-67.
  21. Makarova A.V., Stodola J.L., Burgers P.M. A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis // Nucleic Acids Research, 2012: 40(22), 11618 – 11626.
  22. Smith L.A.*, Makarova A.V.*, Samson L., Thiesen K.E., Dhar A., Bessho T. Bypass of a psoraen DNA interstrand cross-link by DNA polymerases beta, iota, and kappa in vitro // Biochemistry, 2012:51, 8931 – 8938. * equal contribution
  23. Makarova A.V., Kulbachinskiy A.V. Structure of human DNA polymerase iota and the mechanism of DNA synthesis // Biochemistry (Mosc). 2012, 77(6):547-61. *corresponding authors
  24. Lachin A.V., Kazakov A.A., Makarova A.V., Pavlov Y.I., Efremova A.S., Shram S.I., Тarantul V.Z., Gening L.V. Isolation and characterization of high affinity aptamers selected against DNA polymerase iota // Nucleic Acid Ther., 2012: 22 (1), 49 – 57.
  25. Makarova I.V., Kazakov A.A., Makarova A.V., Khaidarova N.V., Kozikova L.V., Nenasheva V.N., Gening L.V., Tarantul V.Z., Andreeva L.E. Transient expression and activity of human DNA polymerase iota in loach embryos // Biotechnology Letters, 2012: 34 (2), 205 – 212.
  26. Makarova A.V., Grabow C., Gening L.V., Tarantul V.Z., Tahirov T.T., Bessho T. Pavlov Y.I. Inaccurate DNA synthesis due to the presence of DNA polymerase iota activity in whole cell extracts // PLOS One, 2011: 6 (1), 1 – 10.
  27. Kazakov A.A., Makarova A.V., Grishina E.E., Tarantul V.Z., Gening L.V. Activity of DNA polymerase iota in human basal cell carcinoma // Curr. Topics Biochem. Res, 2010: 12 (2), 39 – 49.
  28. Макарова А.В., Генинг Л.В., Макарова И.В., Тарантул В.З. Активность склонной к ошибкам ДНК-полимеразы йота в разные периоды отногенеза домовой мыши Mus musculus // Онтогенез, 2008: 39(5), 367 – 373.
  29. Макарова А.В., Генинг Л.В., Тарантул В.З. Эволюция структуры и функции ДНК-полимеразы йота у эукариот // Биохимия, 2008: 73(3), 426 – 433.
  30. Генинг Л.В., Макарова А.В., Малашенко А.М., Тарантул В.З. Фальшивая нота ДНК-полимеразы йота в ансамбле защитников генома млекопитающих // Биохимия, 2006: 71(2), 155 – 159