Lunina Nataliya Aleksandrovna | Institute of Molecular Genetics

Lunina Nataliya Aleksandrovna

Academic degree:
Doctor of Philosophy (Biology)

Academic title:
without academic rank

Division of IMG:
Laboratory of protein engineering




Main research interests

After graduating from the Department of Virology and Doctorate of the Biological Faculty of the Moscow State University she defended her PhD thesis on the topic “Translation of RNA of Barley Stripe Mosaic Virus in a Cell-free System” under the guidance of Academician I.G. Atabekov. Continued working at the Department of Virology, studied RNA-containing plant viruses, including the closterovirus genomic RNA structure. Results of the studies were published in leading virology journals. The total number of publications – 11.
Since March 1998 worked at the Laboratory of the Molecular Genetics of Anaerobes headed by G.A. Velikodvorskaya at the IMG RAS. The main focus of the studies became thermophylic glycoside hydrolyses capable of working at extremely high temperatures (up to 90˚C). There were studied amylase complex ferments, including the cluster of genes involved in the hydrolysis of maltodextrins. Multimodular enzymes were studied: functions of individual modules, including catalitic and carbohydrate-binding, were obtained and studied. A chimeric thermally stable lactase capable of strongly binding to cellulose and hydrolyzing lactose in whey was engineered. A carbohydrate-binding module of a new, previously unknown, family, which is capable of binding to chitin and chitosan was characterized. In this area she has 18 publications and one patent.
Since 2006 she has been a Researcher at the Protein Engineering Laboratory. Participated in research of viral proteases as possible anti-cancer agents, including studies into mechanisms of cell death caused by  protease 3C of hepatitis A virus. 2 works were published on this subject. 

Teaching activities

Taught student to perform experimental work, directed practical training at the Department of Virology of the MSU Faculty of Biology.
Supervised about 10 student graduate thesis and one PhD thesis.  


  1. Dolja V.V., Sokolova (Lunina) N.A., Tjulkina L.G., Atabekov J.G. A study of barley stripe mosaic virus genome. II. Translation of individual RNA species of two BSMV strains in a homologous cell-free system. Mol.Gen.Genet., 1979, 175, 93-97.
  2. Dolja V.V., Lunina N.A., Atabekov J.G.  The study of individual RNA translation four-component strain BSMV in a heterologous cell-free system. Proceedings LSKHA, 1981, 191, 49-52. Russian.
  3. Dolja V.V., Lunina N.A., Smirnov V.D., Karpov V.A., Khudyakov Y.E., Kozlov Y.V., Baev A.A., Atabekov I.G. Localization of genes in the polycistronic mRNA by oligonucleotide probe on the example of the viral coat protein gene of barley stripe mosaic virus. Reports of the USSR Academy of Sciences, 1982, 265, 474-477. Russian.
  4. Baratova L.A., Belyanova L.P., Khodova A.M., Lunina N.A., Dolja V.V., Atabekov I.G. The extended N-terminal amino acid sequence of the structural proteins of the two strains of barley stripe mosaic virus. Biol. Science, 1983, №4, 23-26. Russian.
  5. Dolja V.V., Agranovsky A.A., Lunina N.A., Atabekov J.G. Short virion RNA in barley stripe mosaic virus. FEBS lett., 1983, 151, 215-218.
  6. Dolja V.V., Lunina N.A., Leiser R.-M., Stanarius T., Belzhelarskaya S.N., Kozlov Y.V., Atabekov J.G. A comparative study on the in vitro translation products of individual RNAs of two-, three-, and four-component strains of barley stripe mosaic virus. Virology, 1983, 127, 1-14.
  7.  Dolja V.V., Lunina N.A., Chumakov K.M., Ziegler A., Atabekov I.G. The subgenomic RNA of barley stripe mosaic virus. Reports of the USSR Academy of Sciences, 1986, 288, 999-1001. Russian.
  8.  Dolja V.V., Lunina NA, Karpova O.V., Rodionova N.P., Tyulkina L.G., Kulaeva O.I. Atabekov I.G. The length of an internal poly(A)-block genetically determined for each individual RNA of barley stripe mosaic virus. Proceedings of the Academy of Sciences of the USSR, Biological Series, 1989, №1, 133-137. Russian.
  9. Agranovsky A.A., Boyko V.P., Karasev A.V., Lunina N.A., Koonin E.V., and DoljaV.V. Nucleotide sequence of the 3`-terminal half of beet yellows  closterovirus RNA genome: unique arrangement of eight virus genes. Journal of  General Virology, 1991, 72, 15-23.
  10. Agranovsky A.A., Karasev A.V., Novikov V.K., Lunina N.A., Loginov S., and Tyulkina L.G. Poa semilatent virus, a hordeivirus having no internal polydisperse poly(A) in the 3` non-coding region of the RNA genome. Journal of General Virology, 1992, 73, 2085-2092.
  11. Agranovsky, A.A., Koonin, E.V., Boyko, V.P., Maiss, E., Froetschl, R., Lunina N.A. and Atabekov, J.G. Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease. Virology, 1994, 198, 311-324.
  12. Zverlov V.V., Volkov I.Iu., Lunina N.A., Velikodvorskaya G.A. Enzymes of thermophilic anaerobic bacteria hydrolyze cellulose, xylan and other beta-glucans. Mol Biol (Mosk), 1999, 33(1), 106-112. Russian.
  13. Berezina O.V., Zverlov V.V., Lunina N.A., Chekanovskaya L.A., Dubinina E.N., Libel' V, Velikodvorskaya G.A. Structure and properties of the gene for 4-alpha-glucanotransferase from Thermotoga neapolitana. Mol Biol (Mosk), 1999, 33(5), 905-911. Russian.
  14. Zolotarev Y.A., Dadayan A.K., Borisov Y.A., Dorokhova E.M., Kozik V.S., Vtyurin N.N., Bocharov E.V., Ziganshin R.N., Lunina N.A., Kostrov S.V., Ovchinnikova T.V., Myasoedov N.F. The effect of three-dimensional structure on the solid state isotope exchange of hydrogen in polypeptides with spillover hydrogen. Bioorganical Chemistry, 2003, 31, 453-463.
  15. Veith B., Zverlov V. V., Lunina N.A., Berezina O.V., Raasch C., Velikodvorskaya G.A., and Liebl W. Comparative analysis of the recombinant α-glucosidases from the Thermotoga neapolitana and Thermotoga maritima maltodextrin utilization gene clusters. Biocatalysis and Biotransformation. 2003, 21, 147-158.
  16. Berezina O.V., Lunina N.A., Zverlov V.V., Naumov D.G., Liebl W., Velikodvorskaya G.A. Thermotoga neapolitana gene clusters participating in degradation of starch and maltodextins: molecular structure of the locus. Mol Biol (Mosk), 2003,37(5),801-809. Russian.
  17. Lunina N.A., Berezina O.V., Veith B., Zverlov V.V., Vorob'eva I.P., Chekanovskaya L.A., Khromov I.S., Raash G., Libel' V., Velikodvorskaya G.A. Thermotoga neopolitina gene cluster, participating in degradation of starch and maltodextrins: expression of aglB and aglA gene in Escherichia coli, properties of recombinant enzymes. Mol Biol (Mosk), 2003, 37(5), 810-819. Russian.
  18. Volkov I.Iu, Lunina N.A., Velikodvorskaya G.A. Prospects for practical application of substrate-binding modules of glycosyl hydrolases. Prikl Biokhim Mikrobiol., 2004, 40(5),499-504. Review. Russian.
  19. Volkov I.Iu., Lunina N.A., Berezina O.V., Velikodvorskaya G.A., Zverlov V.V. Thermoanaerobacter ethanolicus gene cluster containing the alpha- and beta-galactosidases genes melA and lacA, and properties of recombinant lacA. Mol Biol (Mosk), 2005, 39(6), 915-922. Russian.
  20. Dvortsov I.A., Lunina N.A., Chekanovskaya L.A., Shedova E.N., Gening L.V., Velikodvorskaya G.A. Ethidium bromide is good not only for staining of nucleic acids but also for staining of proteins after polyacrylamide gel soaking in trichloroacetic acid solution. Analytical Biochemistry, 2006, 253, (2), 293-295.
  21. Lunina N.A., Agafonova E.V., Chekanovskaja L.A., Dvortsov I.A., Berezina O.V., Shedova E.N., Kostrov S.V., Velikodvorskaya G.A. Co-expression of the Thermotoga neapolitana aglB Gene with an Upstream 3’-Coding Fragment of the malG Gene Improves Enzymatic Characteristics of Recombinant AglB Cyclomaltodextrinase. Protein Expression and Purification, 2007, 54, 18-23.
  22. Shedova E.N., Berezina O.V., Lunina N.A., Zverlov V.V., Schwartz W.H., Velikodvorskaya G.A. Cloning and characterization of a large metagenomic DNA fragment containing glycosyl-hydrolase genes. Mol Gen Mikrobiol Virusol., 2009, 1, 11-15. Russian.
  23. Shedova E.N., Lunina N.A., Berezina O.V., Zverlov V.V., Schwarz V., Velikodvorskaya G.A. Expression of the genes CelA and XylA isolated from a fragment of metagenomic DNA in Escherichia coli. Mol Gen Mikrobiol Virusol., 2009, 2, 28-32. Russian.
  24.   Dvortsov I.A., Lunina N.A., Chekanovskaya L.A., Schwarz W.H., Zverlov V.V., Velikodvorskaya G.A. Carbohydrate binding properties of a separately folding protein module from {beta}-1,3-glucanase Lic16A of Clostridium thermocellum. Microbiology, 2009, 155, 2442-2449.
  25. Dvortsov I.A., Lunina N.A., Zverlov V.V., Velikodvorskaya G.A. Substrate-binding properties of family 54 module of laminarinase Lic16A of Clostridium thermocelum. Mol Biol (Mosk), 2010, 44(4), 671-676. Russian.
  26.  Velikodvorskaya G.A.,  Tikhonova T.V., Gurvits I.D., Karyagina A.S., Lavrova N.V., Sergienko O.V., Tashlitskii V.N., Lunina N.A., and Lunin V.G. Сhimeric lactase capable of spontaneous and strong immobilization on cellulose and development of continuous-flow system for lactose hydrolysis at high temperatures,  Appl. and Envir. Microbiol., 2010, 76, 8071-8075.
  27. Shubin A.V., Lunina N.A.,Shedova E.N., Roschina M.P., Demidyuk I.V., Vinogradova T.V., Kopantsev E.P.,Chernov I.P., and Kostrov S.V. Assessment of toxic effect during transient expression of genes for proteases of Human pathogens in HEK293 Line Cells. Biotechnology, 2012, № 4, 82-88. Russian.
  28. Dvortsov I.A., Lunina N.A., Zverlov V.V., Velikodvorskaya G.A. The properties of four C-terminal carbohydrate-binding modules (CBM4) of laminarinase Lic16A of Clostridium thermocellum. Mol Biol (Mosk), 2012, 46(6), 915-921. Russian.
  29. Velikodvorskaya G.A., Chekanovskaya L.A., Lunina N.A., Sergienko O.V., Lunin V.V., Dvortsov I.A., Zverlov V.V. The family 28 carbohydrate-binding module of the thermostable endo-1,4-β-glucanase CelD Caldicellulosiruptor bescii maximizes the enzymes activity and binds irreversibly to amorphous cellulose. Mol. Biol. (Mosk),  2013, 47, №4, 667-673. Russian.
  30. Kislitsyn Y.A., Samygina V.R., Dvortsov I.A., Lunina N.A., Kuranova I.P.  and Velikodvorskaya G.A. Crystallization and preliminary X-ray diffraction studies of the family 54 carbohydrate-binding module from laminarinase (β-1,3-glucanase) Lic16A of Clostridium thermocellum. Acta Cryst., 2015, F71, 217-220.
  31. Shubin A.V., Demidyuk I.V., Lunina N.A., Komissarov A.A., Roschina M.P., Leonova O.G.  and Kostrov S.V. Protease 3C of hepatitis A virus induces vacuolization of lysosomal/endosomal organelles and caspase-independent cell death. BMC Cell Biology, 2015, 16:4, DOI 10.1186/s12860-015-0050-z.
  32. Velikodvorskaya G.A., Zverlov V.V., Karyagina-Zhulina A.S., Lavrova N.V., Lunin V.G., Lunina N.A., Ryazanova E.M., Sergienko O.V., Tikhonova T.V. June 2006. Russian Federation patent 2278160.