Koksharova Olga

Major works are devoted to developing methods for the study of genetics of cyanobacteria, analysis of the structure and functions of genes involved in cell division and the main metabolic processes in the cells of cyanobacteria: nitrogen fixation, cell differentiation, photosynthesis and the formation of toxins.
 
1984-1987. A series of studies on the development of methods for cloning genes of cyanobacterial photosynthesis and herbicide tolerance, and their search for plant homologs.

1987 Obtaining of the cyanobacterium Synechocystis 6803 mutants for the design of the cloning of genes of photosynthesis
Thesis on the specialty 03.02.07 - Genetics (biol. Of Science)
Author: Koksharova OA, Ph.D., Moscow State University Lomonosov
Supervisor: Sergey Shestakov
Defended in the council D 002.49.01 at Moscow State University Lomonosov
The organization in which the work was done: Lomonosov Moscow State University Lomonosov
Lead Organization: Institute of Genetics
Opponents: Ananiev, Matorin DN
 
1988-1993. Studies of Stability of cloned genes in cells of Bacillus subtilis.

1993-1994. Genetic analysis of the controlled light cascade in the cells of the cyanobacterium
Synechocystis PCC 6803. Collaboration with Dr. F.Chauvat, France.

1995-1998. An investigation of highly divergent genes encoding glyceraldehyde-3-phosphate dehydrogenase
 involved in the Calvin cycle and glycolysis in cells of Synechocystis PCC 6803 and Synechococcus PCC 7942.
 Collaboration with Prof. Cerff, Germany ( Koksharova O., Schubert M.,
Shestakov S., Cerff R. Genetic and biochemical evidence for distinct key functions of two highly divergent
 GAPDH genes in catabolic and anabolic carbon flow of the cyanobacterium Synechocystis sp. PCC 6803.
Plant Mol.Biol.,1998, v.36/1,p.183-194).
 
1998-2001. Identification of new DNA-binding proteins that regulate the expression of genes of cell differentiation of cyanobacteria. Collaboration with Professor P.Wolk, USA (Koksharova O.A., Wolk C.P. Novel DNA-Binding Proteins in the Cyanobacterium Anabaena sp. Strain PCC 7120. J Bacteriol. 2002, v.184, p.3931-3940).

2006. Doctoral thesis.
Genetic control division, development and metabolism of the cyanobacterial cells
Author: Koksharova OA, Ph.D., Moscow State University Lomonosov
Scientific adviser: Sergey Shestakov
Defended in the council D 002.214.01 at the Institute of General Genetics, Russian Academy of Sciences
The organization in which the work was done: IOGEN RAS
Lead organization: St. Petersburg State University
Opponents: Los DA, Prozorov AA, IA Khmel
 
2000-2012. A series of papers on the genetics of cell division cyanobacteria. It puts a new direction in the study of genes controlling the division of cyanobacteria and the chloroplasts of higher plants. Proteomics of cell division. The first protein map of cyanobacteria Synechococcus PCC 7942. Collaboration with  U. Rasmussen, Johan Klint, Sweden. (Koksharova O.A., Wolk C.P. A novel gene that bears a DnaJ motif influences cyanobacterial cell division. J. Bacteriol. 2002,v.184, p.5524-5528; Vitha S., Froehlich J.E., Koksharova O., Pyke K..A., van Erp H., Osteryoung K.W. ARC6 is a J-domain plastid division protein and an evolutionary descendant of the cyanobacterial cell division protein Ftn2. Plant Cell. 2003, v15:1918-1933; Koksharova OA, Klint J., Rasmussen U. Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Microbiology (Reading, England), Society for General Microbiology (United Kingdom), 2007 том 153, с. 2505-2517
Koksharova OA, Klint I., Rasmussen U. The first protein map of Synechococcus sp. strain PCC 7942.Mikrobiologiia, издательство Izdatel'stva Nauka (Russian Federation), 2006,том 75, № 6, с. 765-774)

2012-1015. Молекулярное типирование и филогенетический анализ новых цианобактерий -симбионтов беспозвоночных животных и сельскохозяйственных растений.
(2013 Koksharova O.A., Kravzova T.R., Lazebnaya I.V., Gorelova O.A., Baulina O.I., Lazebny O.E., Fedorenko T.A., Lobakova E.S. Molecular Identification and Ultrastructural and Phylogenetic Studies of Cyanobacteria from Association with the White Sea Hydroid Dynamena pumila (L., 1758) BioMed Research International, том 2013, с. 760681 DOI 10.1155/2013/760681; 2015 Karaushu E.V., Lazebnaya I.V., Kravzova T.R., Vorobey N.A., Lazebny O.E., Kiriziy D.A., Olkhovich O.P., Taran N.Yu, Kots S.Ya, Popova A.A., Omarova E., Koksharova O.A. Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and Nodule Sinorhizobium with Medicago sativa L. BioMed Research International DOI 10.1155/2015/202597)

2012-2015. Identification of genes that determine the sensitivity of cyanobacteria to the VOC of bacterial origin.

2011-2015. Study of biogenesis of metal nanoparticles in cyanobacteria.

from 2012- at present time. Study of the nature impact of cyanotoxin BMAA on cyanobacteria cells using genetic and proteomic methods.