Esyunina Daria Мikhailovna | Institute of Molecular Genetics

Esyunina Daria Мikhailovna

Academic degree:
Doctor of Philosophy (Biology)

Academic title:
without academic rank

Division of IMG:
Laboratory of molecular genetics of microorganisms
Laboratory of RNA biology and epigenetics

Senior Researcher


Resume (CV):

Main research interests

Current projects:
1) transcription regulation in cyanobacteria
2) study of small nucleic acid biogenesis by bacterial Argonaute proteins
3) developing plasmid-based system with inducible transcription, translation and replication
4) developing in vivo and in vitro systems to study interactions of RNA-polymerase with other molecular machines
5) investigation of transcription-coupling processes
Partial results in these fields  are present in publications.

Awards, achievements, memberships

2017 – Medal of Russian Academy of Science for young scientists
2014 - best poster prize on 76th Harden Conference Total Transcription, September 2014 – Poster Prize of 76th Harden Conference
2014 - 2016 “The Future of Molecular Genetics” Fellowship, Institute of Molecular Genetics, Moscow, 2003-2005
2010 - M.S. with honors, Moscow State University
2009 - R.B. Khesin prize for undergraduate work
2005 - Silver Medal in the 16th International Biology Olympiad - Beijing, China.


  1. Petushkov I, Esyunina D, Mekler V, Severinov K, Pupov D, Kulbachinskiy A. Interplay between σ region 3.2 and secondary channel factors during promoter escape by bacterial RNA polymerase. Biochem J. 2017 Dec 1;474(24):4053-4064.
  2. Petushkov I, Esyunina D, Kulbachinskiy A. Possible roles of σ-dependent RNA polymerase pausing in transcription regulation. RNA Biol. 2017 Dec 2;14(12):1678-1682.
  3. Miropolskaya N, Esyunina D, Kulbachinskiy A. Conserved functions of the trigger loop and Gre factors in RNA cleavage by bacterial RNA polymerases. J Biol Chem. 2017 Apr 21;292(16):6744-6752.
  4. Agapov A, Olina A, Esyunina D, Kulbachinskiy A. Gfh factors and NusA cooperate to stimulate transcriptional pausing and termination. FEBS Lett. 2017 Mar;591(6):946-953.
  5. Petushkov I, Esyunina D, Kulbachinskiy A. σ38-dependent promoter-proximal pausing by bacterial RNA polymerase. Nucleic Acids Res. 2017 Apr 7;45(6):3006-3016. 
  6. Agapov A, Esyunina D, Pupov D, Kulbachinskiy A. Regulation of transcription initiation by Gfh factors from Deinococcus radiodurans. Biochem J. 2016 Dec 1;473(23):4493-4505.
  7. Esyunina D, Agapov A, Kulbachinskiy A. Regulation of transcriptional pausing through the secondary channel of RNA polymerase. Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):8699-704.
  8. Esyunina D, Turtola M, Pupov D, Bass I, Klimašauskas S, Belogurov G, Kulbachinskiy A. Lineage-specific variations in the trigger loop modulate RNA proofreading by bacterial RNA polymerases. Nucleic Acids Res. 2016 Feb 18;44(3):1298-308.
  9. Esyunina DM, Kulbachinskiy AV. Purification and Characterization of Recombinant Deinococcus radiodurans RNA Polymerase. Biochemistry (Mosc). 2015 Oct;80(10):1271-8. 
  10. Esyunina D, Klimuk E, Severinov K, Kulbachinskiy A. Distinct pathways of RNA polymerase regulation by a phage-encoded factor. Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):2017-22.  
  11. Basu RS, Warner BA, Molodtsov V, Pupov D, Esyunina D, Fernandez-Tornero C, Kulbachinskiy A, Murakami KS. Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme. J Biol Chem. 2014 Aug 29;289(35):24549-59. 
  12. Tagami S, Sekine S, Minakhin L, Esyunina D, Akasaka R, Shirouzu M, Kulbachinskiy A, Severinov K, Yokoyama S. Structural basis for promoter specificity switching of RNA polymerase by a phage factor. Genes Dev. 2014 Mar 1;28(5):521-31. 
  13. Miropolskaya N*, Esyunina D*, Klimasauskas S, Nikiforov V, Artsimovitch I, Kulbachinskiy A. Interplay between the trigger loop and the F loop during RNA polymerase catalysis. Nucleic Acids Res. 2014 Jan;42(1):544-52. 
  14. Pupov D*, Esyunina D*, Feklistov A, Kulbachinskiy A. Single-strand promoter traps for bacterial RNA polymerase. Biochem J. 2013 Jun 1;452(2):241-8. 
  15. Berdygulova Z*, Esyunina D*, Miropolskaya N, Mukhamedyarov D, Kuznedelov K, Nickels BE, Severinov K, Kulbachinskiy A, Minakhin L. A novel phage-encoded transcription antiterminator acts by suppressing bacterial RNA polymerase pausing. Nucleic Acids Res. 2012 May;40(9):4052-63. 
  16. Zhilina E, Esyunina D, Brodolin K, Kulbachinskiy A. Structural transitions in the transcription elongation complexes of bacterial RNA polymerase during sigma-dependent pausing. Nucleic Acids Res. 2012 Apr;40(7):3078-91.