Dergunova Lyudmila Vasilievna | Institute of Molecular Genetics
|
Academic degree:
Doctor of Philosophy (Biology)
Academic title:
without academic rank
Division of IMG:
Laboratory of molecular human genetics
Position:
Senior Researcher
Telephone:
+7-499-196-18-58
Е-mail: |
Main research interests
Main scientific interests are focused on studying the genes active in the brain.
Research is carried out in two areas: the study of structural and functional organization of the SGMS1 gene, encoding the enzyme sphingomyelin synthase 1, and the study of molecular mechanisms of neuroprotective action of peptides in ischemic brain damage.
The structure of SGMS1 gene had been identified, and a number of alternative transcripts had been discovered. It is shown that in the regulation of this gene the mechanisms using alternative promoters, alternative splicing and alternative polyadenylation. In the experimental models of cerebral ischemia in rats it was revealed a number of genes, which is changed expression after application of semax and Pro-Gly-Pro (PGP) neuropeptides. It was shown the effect of synthetic peptides on a range of biological processes that contribute to the functioning of various body systems. For the first time it was revealed semax effect on the immune and vascular system in conditions of ischemic injury.
Research is carried out in two areas: the study of structural and functional organization of the SGMS1 gene, encoding the enzyme sphingomyelin synthase 1, and the study of molecular mechanisms of neuroprotective action of peptides in ischemic brain damage.
The structure of SGMS1 gene had been identified, and a number of alternative transcripts had been discovered. It is shown that in the regulation of this gene the mechanisms using alternative promoters, alternative splicing and alternative polyadenylation. In the experimental models of cerebral ischemia in rats it was revealed a number of genes, which is changed expression after application of semax and Pro-Gly-Pro (PGP) neuropeptides. It was shown the effect of synthetic peptides on a range of biological processes that contribute to the functioning of various body systems. For the first time it was revealed semax effect on the immune and vascular system in conditions of ischemic injury.
Teaching activities
It was prepared more than ten graduate theses.
Dissertation leadership: four PhD theses had been defended, two PhD theses are preparing to defend. Supervisor of postgraduate students: Fillioenkov IB, Kalinichenko EO.
Dissertation leadership: four PhD theses had been defended, two PhD theses are preparing to defend. Supervisor of postgraduate students: Fillioenkov IB, Kalinichenko EO.
Publications
It was published 36 articles in peer-reviewed journals.
List of main publications:
List of main publications:
- Vladychenskaya I.P., Dergunova L.V., Dmitrieva V.G., Limborska S.A. // Human gene MOB: structure specification and aspects of transcriptional activity. // Gene, 2004, v. 338 (2), 257-265.
- Dmitrieva V.G., Torshina E.V., Yuzhakov V.V., Povarova O.V., Skvortsova V.I., Limborska S. A., Dergunova L.V. // Expression of sphingomyelin synthase 1 gene in rat brain focal ischemia.// Brain Res. 2008 Jan 10;1188:222-227
- Dmitrieva V.G, Povarova O.V., Skvortsova V.I, Limborska S.A., Myasoedov N.F., Dergunova L.V. Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia. // Cell Mol Neurobiol. 2010 Jan; 30(1):71-79.
- Stavchansky VV, Yuzhakov VV, Botsina AY, Skvortsova VI, Bondurko LN, Tsyganova MG, Limborska SA, Myasoedov NF, Dergunova LV. // The Effect of Semax and Its C-End Peptide PGP on the Morphology and Proliferative Activity of Rat Brain Cells During Experimental Ischemia: A Pilot Study.// J Mol Neurosci. 2011 Oct;45(2):177-85. doi: 10.1007/s12031-010-9421-2.
- Rozhkova AV, Dmitrieva VG, Zhapparova ON, Sudarkina OY, Nadezhdina ES, Limborska SA, Dergunova LV. // Human sphingomyelin synthase 1 gene (SMS1): Organization, multiple mRNA splice variants and expression in adult tissues // Gene. 2011 Aug 1; 481(2):65-75.
- Dergunova LV, Rozhkova AV, Sudarkina OYu, Limborska SA // The Use of Alternative Polyadenylation in the Tissue-Specific Regulation of Human SMS1 Gene Expression// Mol Biol Rep, 2013, Dec; 40(12):6685-6690.DOI 10.1007/s11033-013-2783-0.
- Medvedeva EV, Dmitrieva VG, Povarova OV, Limborska SA, Skvortsova VI, Myasoedov NF, Dergunova LV. //Effect of semax and its C-terminal fragment Pro-Gly-Pro on the expression of VEGF family genes and their receptors in experimental focal ischemia of the rat brain.//J Mol Neurosci. 2013 Feb; 49(2):328-33. doi: 10.1007/s12031-012-9853-y.
- Medvedeva E.V., Dmitrieva V.G., Povarova O.V., Limborska S.A., Skvortsova V.I., Myasoedov N.F., Dergunova L.V. The peptide semax affects the expression of genes related to the immune and vascular systems in rat brain focal ischemia: Genome-wide transcriptional analysis. BMC Genomics 2014; 15(1): 228. doi: 10.1186/1471-2164-15-228.
- Sudarkina OY, Filippenkov IB, Brodsky IB, Limborska SA, Dergunova LV. Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues. Mol Cell Biochem. 2015 Aug;406(1-2):91-9.
- Filippenkov IB, Sudarkina OY, Limborska SA, Dergunova LV. Circular RNA of the human sphingomyelin synthase 1 gene: Multiple splice variants, evolutionary conservatism and expression in different tissues. RNA Biol. 2015 Sep 2;12(9):1030-42.