Demidyuk Ilya Valerievich | Institute of Molecular Genetics

Demidyuk Ilya Valerievich

Academic degree:
Doctor of Chemistry

Academic title:
Associated Professor

Division of IMG:
Laboratory of protein engineering

Leading Researcher



Main research interests

Chemistry and biology of proteolytic enzymes, protease’s application in biotechnology and medicine

Teaching activities

1998 – 2016 associated professor of the Department of Biotechnology and Industrial Pharmacia, Moscow State University of Fine Chemical Technologies (MITHT)
2005 – teaching at the Scientific and Educational Center of the Institute of Molecular Genetics
2013 – professor of the Department of Nano-, Bio-, Information Technology and Cognitive Science, Moscow Institute of Physics and Technology (State University)
2016 – professor of the Department of Biotechnology and Industrial Pharmacia, Moscow Technological University (MITHT)
Supervisor of 2 Ph.D. students, 3 defended PhD. theses.

Awards, achievements, memberships

Member of Editorial Board of “World Journal of Biological Chemistry”
Member of the Scientific Council, Institute of Molecular Genetics, RAS


  1. Demidyuk I.V., Kalashnikov A.E., Gromova T.Yu., Gasanov E.V., Safina D.R., Zabolotskaya M.V., Rudenskaya G.N., Kostrov S.V. Cloning, sequencing, expression, and characterization of protealysin, a novel neutral proteinase from Serratia proteamaculans representing a new group of thermolysin-like proteases with short N-terminal region of precursor. // Protein Expression and Purification. 2006. V. 47. № 2. P. 551-561.
  2. Smolov M., Gottikh M., Tashlitskii V., Korolev S., Demidyuk I., Brochon J.-C., Mouscadet J.-F., Deprez E. Kinetic study of the HIV-1 DNA 3’-end processing: single-turnover property of Integrase. // FEBS Journal. 2006. V. 273. № 6. P. 1137-1151.
  3. Sharipova M.R., Shagimardanova E.I., Chastukhina I.B., Shamsutdinov T.R., Balaban N.P., Mardanova A.M., Rudenskaya G.N., Demidyuk I.V., Kostrov S.V. The expression of Bacillus intermedius glutamyl endopeptidase gene in Bacillus subtilis recombinant strains. // Molecular Biology Reports. 2007. V. 34. № 2. P. 79-87.
  4. Sharipova M., Balaban N., Kayumov A., Kirillova Y., Mardanova A., Gabdrakhmanova L., Leshchinskaya I., Rudenskaya G., Akimkina T., Safina D., Demidyuk I., Kostrov S. The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis. // Microbiol. Res. 2008. V. 163. № 1. Р. 39-50.
  5. Demidyuk I.V., Gasanov E.V., Safina D.R., Kostrov S.V. Structural organization of precursors of thermolysin-like proteinases. // Protein J. 2008. V. 27. № 6. P. 343-354.
  6. Gasanov E.V., Demidyuk I.V., Shubin A.V., Kozlovskiy V.I., Leonova O.G., Kostrov S.V. Hetero- and auto-activation of recombinant glutamyl endopeptidase from Bacillus intermedius. // Protein Engineering, Design, and Selection. 2008. V. 21. № 11. P. 653-658.
  7. Gromova T.Yu., Demidyuk I.V., Kozlovskiy V.I., Kuranova I.P., Kostrov S.V. Processing of protealysin precursor. // Biochimie. 2009. V. 91. № 5. P. 639-645.
  8. Demidyuk I.V., Gromova T.Y., Polyakov K.M., Melik-Adamyan W.R., Kuranova I.P., Kostrov S.V. Crystal structure of protealysin precursor: insights into propeptide function. // J. Biol. Chem. 2010. V. 285. № 3. P. 2003-2013.
  9. Shubin A.V., Demidyuk I.V., Kurinov A.M., Demkin V.V., Vinogradova T.V., Zinovyeva M.V., Sass A.V., Zborovskaya I.B., Kostrov S.V. Cathepsin D messenger RNA is downregulated in human lung cancer. // Biomarkers. 2010. V. 15. № 7. P. 608-613.
  10. Demidyuk I.V., Shubin A.V., Gasanov E.V., Kostrov S.V. Propeptides as modulators of functional activity of proteases. // Biomolecular concepts. 2010. V. 1. № 3-4. P. 305-322.
  11. Sabirova A.R., Rudakova N.L., Balaban N.P., Ilyinskaya O.N., Demidyuk I.V., Kostrov S.V., Rudenskaya G.N., Sharipova M.R. A novel secreted metzincin metalloproteinase from Bacillus intermedius. // FEBS Lett. 2010. V. 584. № 21. P. 4419-25.
  12. Bozhokina E.S., Tsaplina O.A., Efremova T.N., Kever L.V., Demidyuk I.V., Kostrov S.V., Adam T., Komissarchik Ya.Yu., Khaitlina S.Yu. Bacterial invasion of eukaryotic cells can be mediated by actin-hydrolyzing metalloproteases grimelysin and protealysin. // Cell biology international. 2011. V. 35. № 2. P. 111-118.
  13. Safina D., Rafieva L., Demidyuk I., Gasanov E., Chestukhina G., Kostrov S. Involvement of propeptides in formation of catalytically active metalloproteinase from Thermoactinomyces sp. // Protein and Peptide Letters. 2011. V. 18. № 11. P. 1119-1125.
  14. Klykov S.P., Kurakov V.V., Vilkov V.B., Demidyuk I.V., Gromova T.Y., Skladnev D.A. A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation. // Biofabrication. 2011. V. 3. № 4. P. 045006.
  15. Tsaplina O., Efremova T., Demidyuk I., Khaitlina S. F-actin is a substrate for protealysin, a metalloprotease of invasive Serratia proteamaculans. // FEBS Journal. 2012. V. 279. № 2. P. 264-274.
  16. Demidyuk I.V., Shubin A.V., Gasanov E.V., Kurinov A.M., Demkin V.V., Vinogradova T.V., Zinovyeva M.V., Sass A.V., Zborovskaya I.B., Kostrov S.V. Alterations in gene expression of proprotein convertases in human lung cancer have a limited number of scenarios. // PLoS ONE. 2013. V. 8. № 2. P. e55752.
  17. Demidyuk I.V., Gromova T.Y., Kostrov S.V. Protealysin. / In: Handbook of proteolytic enzymes, 3rd Edition. Edited by Neil D. Rawlings and Guy Salvesen. // Oxford: Academic Press, 2013. V. 1. P. 597-602.
  18. Mikhailova A.G., Khairullin R.F., Demidyuk I.V., Kostrov S.V., Grinberg N.V., Burova T.V., Grinberg V.Y., Rumsh L.D. Cloning, sequencing, expression, and characterization of thermostability of oligopeptidase B from Serratia proteamaculans, a novel psychrophilic protease. // Protein Expression and Purification. 2014. V. 93. P. 63-76.
  19. Shubin A.V., Demidyuk I.V., Lunina N.A., Komissarov A.A., Roschina M.P., Leonova O.G., Kostrov S.V. Protease 3C of hepatitis A virus induces vacuolization of lysosomal/endosomal organelles and caspase-independent cell death. // BMC Cell Biology. 2015. V. 16. P. 4.
  20. Demidyuk I.V., Gromova T.Yu., Kostrov S.V. The propeptide is required for in vivo formation of active protealysin. // Protein and Peptide Letters. 2015. V. 22. № 6. P. 509-513.
  21. Shubin A.V., Demidyuk I.V., Komissarov A.A., Rafieva L.M., Kostrov S.V. Cytoplasmic vacuolization in cell death and survival. // Oncotarget. 2016. V. 7. № 34. P. 55863-55889.