Laboratory of the genome replication and reparation | Institute of Molecular Genetics

Tarantul Vyacheslav Zalmanovich

   
   

List of staff

Tarantul Vyacheslav Zalmanovich, Doctor of Science, Professor.
Gening Leonid Vladimirovich, senior researcher, candidate of biological sciences.
Nenasheva Valentina Valer’evna, senior researcher, candidate of biological sciences.
Makarova Irina Vladimirovna, researcher, candidate of biological sciences.
Kazachenko Konstantin Yur’evich researcher, candidate of biological sciences. 
Kovaleva Galina Vasil’evna, engineer.

   
   

Main directions of research

- Participation incorrect DNA polymerases in replication and repair of the genome.
- The role of trim14 gene in the differentiation and malignant transformation of the cells and in the innate immune response.

   
   

Main scientific results

  1. In the clinic for patients with lower limb ischemia shown positive therapeutic effect of recombinant adenovirus containing the human angiogenin gene.
  2. Obtained and described in detail the aptamer to the DNA polymerase iota human which effectively inhibits the activity of this enzyme).
  3. Developed a new testing method is highly erroneous activity of DNA polymerase iota.
  4. Showed that cadmium ions inhibit the activity of DNA polymerase iota. Cadmium ions significantly reduced the toxic effect of manganese on the nervous system cells. The findings suggest that one reason for the neurotoxic effect of manganese on organisms (manganism) may be due to increase in cells the incorrect enzymatic activity of Poli.
  5. Detected the alternative splicing of mRNA encoded by the gene polymerase iota (Poli). It is shown that without this amino acid sequence encoded by the 2nd exon Poli does not possess DNA polymerase activity in extracts of yeast cells.
  6. Using yeast dihybrid system identified protein partners neuroprotective peptide humanin).
  7. Found and described in detail the site the beginning of replication in the human genome which is located in the 3’-flanking region of the gene ALPHA2-interferon (ori-α2-INF).
  8. Found that transfecting DNA is frequently inserted near DNA replication origins.
  9. It is shown that the TRIM14 gene plays an important role in the antiviral innate immune system.​
    
    

Publications

  1. Tarantul V.Z., Nikolaev A.I., Martinenko A.V., Hannig H., Hunsmann G., Bodemer W. Differential gene expression in B cell non-Hodgkin’s lymphoma of SIV-infected monkey. AIDS Res. Hum. Retroviruses, 2000, 16, N 2, 173-179.
  2. Tarantul V.Z., Hunsmann G. Mitochondrial polypeptides of the oxidative phosphorylation pathway as potential new targets for anti-cancer therapy. Medical Hypotheses, 2001, 56, 386-387.
  3. Tarantul V.Z, Nikolaev A.I, Hannig H., Kalmyrzaev B.B,  Muchoyan I., Maximov V.V, Nenasheva V.V., Dubovaya V.I., Hunsmann G., Bodemer W. Detection of abundantly transcribed genes and gene translocation in human immunodeficiency virus associated non-Hodgkin's lymphoma. Neoplasia, 2001, 3, N 2, 132-142.
  4. Maximov V.V., Hunsmann G., Tarantul V.Z. Mitochondrial 16S rRNA gene encodes a functional peptide, a potential drug for Alzheimer's disease and target for cancer therapy. Medical Hypotheses, 2002, 59, N 6, 670-673.
  5. Shugurova I.M., Bobrisheva I.А., Grivennikov I.A., Tarantul V.Z. The expression of HIV-1 tat and nef genes induces cell-specific changes in growth properties and morphology of different types of rat cells. Cell Proliferation, 2002, 35, N 4, 237-245.
  6. Tarantul V.Z. “Human genome”, 2003, «Languages of Slavic culture», М., 396 с. Монография.
  7. Tarantul V.Z. Transgenic mice as an in vivo model of lymphomagenesis. Int. Rev. Cytology, 2004, 236, 123-180.
  8. Tarantul V.Z. Тарантул В.З. His name is AIDS, М., «Languages of Slavic cultures», 2005, 400 стр. Монография.
  9. Nenasheva V.V., Nikolaev A.I., Martynenko A.V., Kaplanskaya I. B., Hunsmann G., Bodemer W., Tarantul V.Z. Differential gene expression in HIV/SIV-associated and spontaneous lymphomas. Int. J. Med. Sci., 2005, 2, 122-128.
  10. Генинг Л.В., Макарова А.В., Малашенко А.М., Тарантул В.З. Фальшивая нота ДНК-полимеразы йота в ансамбле сторожей генома млекопитающих. Биохимия, 2006, 71, 201-207.
  11. Gening L.V., Tarantul V.Z. Involvement of DNA polymerase iota in hypermutagenesis and tumorigenesis: an improper model can lead to inaccurate results. Immunol. Letters, 2006, 106, 198-199.
  12. Tarantul V.Z. Virus-associated lymphomagenesis. International J. Biomed. Sci., 2006, 2, 101-113.
  13. Avdeeva S.V., Chernukha M.U., Shaginyan I.A., Tarantul V.Z., Naroditsky B.S. Human angiogenin lacks specific antimicrobial activity. Current Microbiology, 2006, 53, 477-478.
  14. Manuilova E.S., Arsenyeva E.L., Khaidarova N.V., Shugurova I.M., Inozemtseva L.S., Tarantul V.Z., Grivennikov I.A. Different effects of regulatory genes (tat, nef) of human immunodeficiency virus type 1 (HIV-1) on the proliferation and differentiation of mouse embryonic stem cells in vitro. International J. Biomed. Sci., 2008, 4, 100-108.
  15. Kholodii G., Dantsevich O., Tarantul V. New origins of DNA replication identified near the human IFNA2 gene. Cell Cycle, 2008, 7, № 24, 40-42.
  16. Tarantul V.Z. Biotechnological explanatory dictionary (Russian-English). М., “Languages of Slavic cultures”, 2009, 936 стр.
  17. Kholodii G., Dantsevich O., Korobov G., Tarantul V. Cell type-specific regulation of a replication origin residing within an AT-rich/LINE-1-rich isochore. Cell Cycle, 2009, 8, № 24, 4176-4178.
  18. Makarova A.V., Grabow C., Gening L.V., Tarantul V.Z., Tahirov T.H., Bessho T., Pavlov Y.I. Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota. PLoS One, 2011, 6(1), e16612.
  19. Makarova I.V., Kazakov A.A., Makarova A.V., Khaidarova N.V., Kozikova L.V., Nenasheva V.V., Gening L.V., Tarantul V.Z., Andreeva L.E. Transient expression and activity of human DNA polymerase iota in loach embryos. Biotechnol. Letters, 2012, 34, 205-212.
  20. Lakhin A.V., Kazakov A.A., Makarova A.V., Pavlov Y.I., Efremova A.S., Shram S.I., Tarantul V.Z., Gening L.V. Isolation and characterization of high affinity aptamers against DNA polymerase iota. Nucleic Acid Ther. 2012, 22, 49-57.
  21. Novosadova E., Khaydarova N., Manuilova E., Arsenyeva E., Lebedev A., Tarantul V., Grivennikov I. Similar effects of mouse and human pub gene on proliferation and embryoid bodies formation in mouse embryonic stem cells in vitro. Stem Cell Discovery, 2012, 2, 78-83.
  22. Kholodii G., Dantsevich O., Tarantul V. Transfecting DNA inserts frequently near replicators. Cell Cycle, 2012, 11 (15), 2956-2958.
  23. Maximov V.V., Martynenko A.V., Arman I.P., Tarantul V.Z. Humanin Binds MPP8: mapping interaction sites of the peptide and the protein. J. Peptide Science, 2013, 19, №5, 301-307.
  24. Nenasheva V.V., Kovaleva G.V., Khaidarova N.V., Novosadova E.V., Manuilova E.S., Antonov A.A., Tarantul V.Z. Trim14 overexpression causes the same transcriptional changes in mouse embryonic stem cells and human HEK293 cells. In Vitro Cellular & Developmental Biology – Animal, 2014, 50, №2, 121-128.
  25. Nenasheva V.V., Kovaleva G.V., Uryvaev L.V., Ionova K.S., Dedova A.V., Vorkunova G.K., Chernyshenko S.V., Khaidarova N.V., Tarantul V.Z. Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity. Immunologic Res., 2015, 62, 255-262. DOI 10.1007/s12026-015-8653-1.
  26. Gening L.V., Lakhin A.V., Makarova I.V., Nenasheva V.V., Andreeva L.E., Tarantul V.Z. Alterations in synthesis and repair of dna during the development of loach Misgurnus fossilis. J. Develop. Biol., 2016, 4, doi:10.3390/jdb4010006.
  27. Kazachenko K.Yu., Miropolskaya N.A., Gening L.V., Tarantul V.Z., Makarova A.V. Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro. DNA Repair, 2017, 50, 77-82. DOI: 10.1016/j.dnarep.2017.01.001. IF - 3.93.
  28. Nenasheva V.V., Novosadova E.V., Makarova I.V., Lebedeva O.S., M. A. Grefenshtein, E. L. Arsenyeva, S. A. Antonov, Grivennikov I.A., Tarantul V.Z. The Transcriptional Changes of trim Genes Associated with Parkinson’s Disease on a Model of Human Induced Pluripotent Stem Cells. Molecular Neurobiology, 2017, 54(9), 7204-7211. DOI: 10.1007/s12035-016-0230-7. IF - 6.19.